Cytotoxicity of substances leached or dissolved from pulp capping materials (2005)
- Authors:
- Autor USP: MARQUES, MARCIA MARTINS - FO
- Unidade: FO
- DOI: 10.1111/j.1365-2591.2005.00967.x
- Subjects: ADESIVOS DENTINÁRIOS; HIDRÓXIDO DE CÁLCIO; CULTURA DE CÉLULAS; CAPEAMENTO DA POLPA DENTÁRIA
- Language: Inglês
- Abstract: AIM: To evaluate the cytotoxic effects of substances leached or dissolved from pulp capping materials on human pulp fibroblasts. METHODOLOGY: The substances were applied to cell cultures in conditioned media. The experimental groups were: GI (control; n = 24)--cultures treated with fresh medium; GII (n = 24)--cultures treated with calcium hydroxide cement; GIII (n = 24)--cultures treated with adhesive resin and GIV (n = 24)--cultures treated with 37% orthophosphoric acid. The media were conditioned by placing the crude materials in contact with fresh culture medium for 1 h. The cytotoxicity analysis was performed using the Trypan blue dye exclusion assay at times of 0, 6, 12 and 24 h for cell viability assay, and at 1, 3, 5 and 7 days for survival assay. Data were treated by anova (P < 0.05) and Tukey's test (P < 0.05). RESULTS: GI and II presented similar cell viability and cell growth. GIII and IV exhibited statistically significant lower percentages of cell viability: GIV only at the 0 h experimental time, whereas in GIII this viability markedly diminished reaching values of 10% by 12 h. Cell growth was impaired only in cultures of GIII. CONCLUSIONS: Substances dissolved from the adhesive system tested were cytotoxic for human dental pulp fibroblasts in culture, whilst substances leached from calcium hydroxide were biocompatible
- Imprenta:
- Source:
- Título: International Endodontic Journal
- ISSN: 0143-2885
- Volume/Número/Paginação/Ano: v. 38, n. 8, p. 505-509, 2005
- Este artigo NÃO possui versão em acesso aberto
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Status: Nenhuma versão em acesso aberto identificada -
ABNT
CAVALCANTI, B. N. e RODE, Sigmar de Mello e MARQUES, Márcia Martins. Cytotoxicity of substances leached or dissolved from pulp capping materials. International Endodontic Journal, v. 38, n. 8, p. 505-509, 2005Tradução . . Disponível em: https://doi.org/10.1111/j.1365-2591.2005.00967.x. Acesso em: 17 mar. 2026. -
APA
Cavalcanti, B. N., Rode, S. de M., & Marques, M. M. (2005). Cytotoxicity of substances leached or dissolved from pulp capping materials. International Endodontic Journal, 38( 8), 505-509. doi:10.1111/j.1365-2591.2005.00967.x -
NLM
Cavalcanti BN, Rode S de M, Marques MM. Cytotoxicity of substances leached or dissolved from pulp capping materials [Internet]. International Endodontic Journal. 2005 ; 38( 8): 505-509.[citado 2026 mar. 17 ] Available from: https://doi.org/10.1111/j.1365-2591.2005.00967.x -
Vancouver
Cavalcanti BN, Rode S de M, Marques MM. Cytotoxicity of substances leached or dissolved from pulp capping materials [Internet]. International Endodontic Journal. 2005 ; 38( 8): 505-509.[citado 2026 mar. 17 ] Available from: https://doi.org/10.1111/j.1365-2591.2005.00967.x - Acceptance and efficiency of anesthesia by photobiomodulation therapy during conventional cavity preparation in permanent teeth: a pilot randomized crossover clinical study
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