Gene expression profiling of selerenewing stem cells from human umbilical cord blood: effects of estradiol (2004)
- Authors:
- Autor USP: MOREIRA FILHO, CARLOS ALBERTO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Umbilical cord blood (UCB) is an alternative source of stem cells currently employed for hematopoietic reconstitution. However, the small amount of hematopoietic stem cells (HSC) available limits transplantation into adults. In this study, the effects of 17-beta-estradiol (E2) on self-renewal of purified CD34+/CD133+ cells were investigated for ex vivo expansion purpose. Total functional HSC increased 14-times after four weeks-cultivation in stroma-free media containing Flt-3L, SCF, IL-6, and IL-3, as indicated by clonogenic assays. Treatment with 10nM E2 further stimulated self-renewal by a factor of 2.5 and did not alter significantly the proportion of erythroid, granulocytic, and megakaryocytic progenitors. DNAmicroarray analysis of 20,000 human genes revealed 388 differentially expressed genes (?3 fold) after E2-treatment (176 up- and 212 down-regulated), classified in 14 major functional categories (27% of unknown function or without geneontology classification). Microarray data was validated by real-time RTPCR of selected genes, such as hTERT, TKS, TRF1, and POT, involved in telomere maintenance. Our data indicates that E2 is effective in supporting self-renewal of HSC in vitro while keeping to some extent their undifferentiated state. Of particular interest was the activation of genes participating in cell cycle, metabolism and mitochondrial transport, in association with repression of genes regulating telomere degradation and apoptosis
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
-
ABNT
CARVALHO, Ana Carolina S. R. et al. Gene expression profiling of selerenewing stem cells from human umbilical cord blood: effects of estradiol. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 11 out. 2024. -
APA
Carvalho, A. C. S. R., Marti, L. C., Tobo, P. R., Moreira-Filho, C. A., & Okamoto, O. K. (2004). Gene expression profiling of selerenewing stem cells from human umbilical cord blood: effects of estradiol. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Carvalho ACSR, Marti LC, Tobo PR, Moreira-Filho CA, Okamoto OK. Gene expression profiling of selerenewing stem cells from human umbilical cord blood: effects of estradiol. Abstracts. 2004 ;[citado 2024 out. 11 ] -
Vancouver
Carvalho ACSR, Marti LC, Tobo PR, Moreira-Filho CA, Okamoto OK. Gene expression profiling of selerenewing stem cells from human umbilical cord blood: effects of estradiol. Abstracts. 2004 ;[citado 2024 out. 11 ] - Ação da testosterona em linfócitos pha-estimulados: estudo de feminização testicular
- Caracterizacao funcional de anticorpos monoclonais para linfocitos ativados de camundongos nude
- Familial cases of xx males: argument for a common origin of y negative 46 xx male and true hermaphrodites
- Reatividade de anticorpos monoclonais especificos para epitopos da membrana externa de n.Meningitidis com antigenos de diferentes sorogrupos desta bacteria
- Estudo do gene sry na intersexualidade humana
- Identificacao do sexo de embrioes bovinos e murinos por metodos imunologicos
- Separacao de espermatozoides portadores do cromossomo x daqueles portadores do y em bovinos por centrifugacao em gradiente de densidade: dados preliminares
- Xy gonadal dysgenesis associated with skeletal alterations: clinical and molecular studies
- Obtenção de marcadores moleculares do tipo RAPD para precocidade reprodutiva em touros da raça nelore
- Estudo genético da reversão sexual XX em suínos: análise de genes candidatos por marcadores do tipo microssatélite
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
