Migration of mouse trophoblast cells in the presence of extracellular matrix components TGF beta (2004)
- Authors:
- Autor USP: BEVILACQUA, ESTELA MARIS ANDRADE FORELL - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Mouse trophoblast cells display an invasive behavior when they penetrate into the endometrium during embryo implantation and the early stages of placentation, coming into contact with stromal cells and extracellular matrix (ECM). The interaction with the ECM is thought to participate in the regulatory mechanisms of trophoblast differentiation, proliferation and migration. In the present work , we have tested how different substrata induce changes in the behavior of implanting trophoblast cells (TC). For this, embryos were collected on the 3,5 th day of pregnancy and plated onto laminin (LN), acid hialuronic (AH), matrigel (M) in Eagles"s medium containing 10% FN-free fetal calf serum and incubated for 72 h. Blatocysts were also poured in traswells containing collagen with LN,AH and M in Eagle"s medium, overnight to watch for invasivness. Cells growth was determined by counting the cells number and the amount of DNA every 72h. The migration of TC cells was estimated by measuring the total area occupied by the growth blastocyst and the invasivness was observed by the number of blastocysts collected from the traswells after 24 h. We have found significant changes in the pattern of migration and invasivness of TC in the presence of LN. This may indicate that LN can play an important role in TC spatial arrangement for implantation during pregnancy
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
GONÇALVES, Claudia Regina e CONTI, Flavia e BEVILACQUA, Estela Maris Andrade Forell. Migration of mouse trophoblast cells in the presence of extracellular matrix components TGF beta. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 06 nov. 2024. -
APA
Gonçalves, C. R., Conti, F., & Bevilacqua, E. M. A. F. (2004). Migration of mouse trophoblast cells in the presence of extracellular matrix components TGF beta. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Gonçalves CR, Conti F, Bevilacqua EMAF. Migration of mouse trophoblast cells in the presence of extracellular matrix components TGF beta. Abstracts. 2004 ;[citado 2024 nov. 06 ] -
Vancouver
Gonçalves CR, Conti F, Bevilacqua EMAF. Migration of mouse trophoblast cells in the presence of extracellular matrix components TGF beta. Abstracts. 2004 ;[citado 2024 nov. 06 ] - Experimental infection of trophoblastic cells from calomys callosus (rodentia: cricetidae) by toxoplasma gondii
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