In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts (2004)
- Authors:
- Autor USP: ARAUJO, VERA CAVALCANTI DE - FO
- Unidade: FO
- DOI: 10.1111/j.1365-2591.2004.00864.x
- Subjects: POLPA DENTÁRIA; PERIODONTO DE PROTEÇÃO; PROTEÍNAS
- Language: Inglês
- Abstract: AIM: To compare the expression of extracellular matrix (ECM) components in human pulpal and gingival fibroblasts in vitro. METHODOLOGY: Cultured dental pulp fibroblasts and gingival mucosa fibroblasts were used. Tenascin (TN), fibronectin (FN), type I (col I) and III collagen (col III) and osteonectin (ONEC) were detected by immunofluorescence. Main morphological characteristics were also analysed by light microscopy (LM) and transmission electron microscopy. RESULTS: The results revealed different expression patterns of the proteins. TN and ONEC were only immunoexpressed by pulpal fibroblast cells, suggesting a role of these glycoproteins in formation of mineralized tissues. FN and col I were present in the cytoplasms of both cell types. No expression of col III was detected. Different morphological characteristics were visualized under LM, in which pulpal fibroblasts were spindle-shaped with a wide cytoplasm, while gingival fibroblast cells exhibited stellate/pyramidal configuration, with rounded nuclei. However, ultrastructurally, both cell lineages showed very well developed rough endoplasmatic reticulum and Golgi complex. CONCLUSIONS: Due to the immunodetection of TN and ONEC on pulpal fibroblasts, the present findings demonstrated that a pulpal fibroblast cell is similar to an osteoblastic cell rather than an undifferentiated mesenchymal cell, such as a gingival fibroblast cell. Functional differences between the two cell lines may then be suggested
- Imprenta:
- Source:
- Título do periódico: International Endodontic Journal
- ISSN: 0143-2885
- Volume/Número/Paginação/Ano: v. 37, n. 11, p. 749-755, November 2004
- Este periódico é de assinatura
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: bronze
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ABNT
MARTINEZ, E. F. e ARAÚJO, Vera Cavalcanti de. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts. International Endodontic Journal, v. 37, n. 11, p. 749-755, 2004Tradução . . Disponível em: https://doi.org/10.1111/j.1365-2591.2004.00864.x. Acesso em: 28 mar. 2024. -
APA
Martinez, E. F., & Araújo, V. C. de. (2004). In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts. International Endodontic Journal, 37( 11), 749-755. doi:10.1111/j.1365-2591.2004.00864.x -
NLM
Martinez EF, Araújo VC de. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts [Internet]. International Endodontic Journal. 2004 ; 37( 11): 749-755.[citado 2024 mar. 28 ] Available from: https://doi.org/10.1111/j.1365-2591.2004.00864.x -
Vancouver
Martinez EF, Araújo VC de. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts [Internet]. International Endodontic Journal. 2004 ; 37( 11): 749-755.[citado 2024 mar. 28 ] Available from: https://doi.org/10.1111/j.1365-2591.2004.00864.x - Estudo histopatológico da reação do tecido subcutâneo de camundongos submetidos a ação de pastas e medicamentos propostos na terapia pulpar de dentes deciduos
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Informações sobre o DOI: 10.1111/j.1365-2591.2004.00864.x (Fonte: oaDOI API)
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