A stereological analysis of the heterotopic osteogenesis induced by allogenic bone matrix grafts in rat subcutaneous tissue (2004)
- Authors:
- USP affiliated authors: ASSIS, GERSON FRANCISCO DE - FOB ; TAGA, RUMIO - FOB
- Unidade: FOB
- Subjects: ENXERTO ÓSSEO; OSTEOGÊNESE; MORFOMETRIA; RATOS
- Language: Inglês
- Abstract: The subcutaneous cellular and tissue alterations that occur during heterotopic osteogenesis induced by a demineralized bone matrix graft in rats were studied mo phometrically. Diaphysis segments obtained from 42 rat femurs were cleaned and demincralized with 0.6 M HCI1. Each segment was Filled with a blood clot and implanted into the back of rats (1 segmentirat). Histological specimens were collected 2, 5, 7, 14, 21, 28 and 49 days after implantation (6 rats, period) and processed after demineralization. Qualitative and morphometric analysis of the histological sections showed that the external blood clots and those filling the medullary canal were replaced with connective tissue within 2-5 days post-implantation. After 14 days, 11.2% and 0.5% of the implanted matrix was replaced with areas of resorption and hyaline cartilage, respectively. After 21 days, 13.7%, 2.4% and 2.7% of the graft matrix showed areas of resorption, cartilage tissue and hone tissue. respectively. At the end of the 49-day study period, 20.2%, 7.8", and 14.5% of the matrix was replaced with areas of resorption, bone tissue and hematopoictic tissue, respectively. Bone induction in rat subcutaneous tissue occurred only at the site of allogenic matrix implantation. The quantity of neoformed cartilage, hone and myeloid tissue depended on the volume of matrix implanted and on the microenvironmental conditions, and was regulated temporally by a cascade of events mediated by bonemorphogenetic proteins (BMPs) presents in the graft. These results also show that morphometric methods may be useful for assessing osteoinduction at ectopic sites
- Imprenta:
- Source:
- Título do periódico: Brazilian Journal Morphological Sciences
- ISSN: 0102-9010
- Volume/Número/Paginação/Ano: v. 21, n. 2, p. 81-88. 2004
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ABNT
HAMATA, Marcelo Matida et al. A stereological analysis of the heterotopic osteogenesis induced by allogenic bone matrix grafts in rat subcutaneous tissue. Brazilian Journal Morphological Sciences, v. 21, n. 2, p. 81-88. 2004, 2004Tradução . . Acesso em: 19 abr. 2024. -
APA
Hamata, M. M., Yunomae, A., Cestari, T. M., Assis, G. F. de, & Taga, R. (2004). A stereological analysis of the heterotopic osteogenesis induced by allogenic bone matrix grafts in rat subcutaneous tissue. Brazilian Journal Morphological Sciences, 21( 2), 81-88. 2004. -
NLM
Hamata MM, Yunomae A, Cestari TM, Assis GF de, Taga R. A stereological analysis of the heterotopic osteogenesis induced by allogenic bone matrix grafts in rat subcutaneous tissue. Brazilian Journal Morphological Sciences. 2004 ; 21( 2): 81-88. 2004.[citado 2024 abr. 19 ] -
Vancouver
Hamata MM, Yunomae A, Cestari TM, Assis GF de, Taga R. A stereological analysis of the heterotopic osteogenesis induced by allogenic bone matrix grafts in rat subcutaneous tissue. Brazilian Journal Morphological Sciences. 2004 ; 21( 2): 81-88. 2004.[citado 2024 abr. 19 ] - Estudo estereológico da glândula parótida do rato durante as 24 horas de um dia de verão
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