Beneficial effect of glutamine on exercise-induced apoptosis of rat neutrophils (2004)
- Authors:
- Autor USP: CURI, RUI - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Português
- Abstract: Objetivo: The effect of a single bout of intensive exercise on apoptosis of rat neutrophils and the possible prevention by glutamine administration was examined. The experiments were performed in sexually immature and sexually mature male rats as to examine the possible involvement of sexual maturation in the effect of exercise. Métodos e Resultados: Exercise was carried out on a treadmill for one hour before rats were killed by decapitation. Aqueous solution of glutamine was given by gavage (1g.kg-1 b.w.), one hour before exercise. Neutrophils were obtained by intraperitoneal lavage with PBS, four hours after injection of oyster glycogen solution. The cells were then analysed for apoptosis by flow cytometry and fluorescence microscopy. Pro- and anti-apoptotic gene expression was evaluated by reverse transcriptase chain reaction (RT-PCR).Neutrophils obtained from immature and mature exercised rats showed an increase in DNA fragmentation, chromatin condensation and phosphatidylserine externalization. This suggests that all neutrophils suffered apoptosis. To study the possible mechanism involved, the production of reactive oxygen metabolites, expression of genes involved in apoptosis and mitochondrial transmembrane potential were examined. Acute exercise raised reactive oxygen metabolites production by neutrophils. Exercise did not change the expression of anti-apoptotic (bcl-xL), and apoptotic (bax and bcl-xS) genes in neutrophils from immature rats but caused a significant increase of bax and bcl-xS expression and provoked a significant decrease of bcl-xL expression in cells from mature rats.Exercise also induced a marked loss of mitochondrial depolarization in neutrophils. Oral glutamine supplementation partially prevented the exercise-induced apoptosis in neutrophils from sexually immature and mature rats. Conclusões: The protective effect of glutamine on neutrophil apoptosis induced by acute exercise possibly occurs by preservation of mitochondrial function.
- Imprenta:
- Publisher place: Águas de Lindóia
- Date published: 2004
- Source:
- Título: Resumos
- Conference titles: Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
PITHON-CURI, Tania Cristina e LAGRANHA, C. J. e CURI, Rui. Beneficial effect of glutamine on exercise-induced apoptosis of rat neutrophils. 2004, Anais.. Águas de Lindóia: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 05 jun. 2025. -
APA
Pithon-Curi, T. C., Lagranha, C. J., & Curi, R. (2004). Beneficial effect of glutamine on exercise-induced apoptosis of rat neutrophils. In Resumos. Águas de Lindóia: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Pithon-Curi TC, Lagranha CJ, Curi R. Beneficial effect of glutamine on exercise-induced apoptosis of rat neutrophils. Resumos. 2004 ;[citado 2025 jun. 05 ] -
Vancouver
Pithon-Curi TC, Lagranha CJ, Curi R. Beneficial effect of glutamine on exercise-induced apoptosis of rat neutrophils. Resumos. 2004 ;[citado 2025 jun. 05 ] - Toxicidade de ácidos graxos em células produtoras de insulina HIT-T15
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