Combined immunization prtocol based on priming with parenteral DNA vaccine and boosting with oral attenuated Salmonella strain confers protective immunity to enterotoxigenic Escherichia coli in a murine model (2003)
- Authors:
- Autor USP: FERREIRA, LUIS CARLOS DE SOUZA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: In this work we evaluated the immunogenicity and protective efficacy of combined priming/boosting protocol based on the structural subunit (CfaB) of the CFA/I fimbriae of enterotoxigenic Escherichia coli (ETEC), which mediates attachment of bacteria to the human intestinal epithelium, encoded by a DNA vaccine and a recombinant attenuated Salmonella Typhimurium aroA strain (HG3). BALB/c mice were i.m. immunized with 2 doses (100 µg/dose) of the DNA vaccine (pRECFA) and after 4 weeks orally boosted with 2 doses (1010 cfu) of the HG3 strain, with or without addition of (25 µg/dose) of a mucosal adjuvant (LTR192G). Our results show that animals submitted to the prime/boost vaccine regimen, either with or without the adjuvant, induced a clear synergic response both for systemic (IgG) and intestinal secreted (sIgA) antibodies. Moreover, sera of mice submitted to the vaccine consortium inhibited the adhesion of CFA/I+ bacteria as demonstrated by in vitro and in vivo tests carried out with Caco-2 cells and using a newborn mice challenge assay, respectively. CFA/I-specific IgA antibodies were also detected in colostrums ingested by newborn mice fostered by vaccinated DBA2 females. Finally, we detected complete (100%) protection to lethal challenge (2 x 107 cfu) with a virulent CFA/I+ ETEC strain given to newborn suckling mice following immunization of pregnant female mice with the complete priming/boosting protocol but not with each vaccine strategies alone. Thiswork shows that the DNA vaccine efficiently boosted mucosal antibody response following oral stimulation with attenuated Salmonella cells and represent a promising alternative for the development of new vaccine protocols against enteric pathogens
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference titles: Congresso Brasileiro de Microbiologia
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ABNT
LASARO, M. O. et al. Combined immunization prtocol based on priming with parenteral DNA vaccine and boosting with oral attenuated Salmonella strain confers protective immunity to enterotoxigenic Escherichia coli in a murine model. 2003, Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. . Acesso em: 01 out. 2024. -
APA
Lasaro, M. O., Sbrogio-Almeida, M. E., Luiz, W. B., Nishimura, L., Guth, B. E. C., & Ferreira, L. C. de S. (2003). Combined immunization prtocol based on priming with parenteral DNA vaccine and boosting with oral attenuated Salmonella strain confers protective immunity to enterotoxigenic Escherichia coli in a murine model. In . Sociedade Brasileira de Microbiologia. -
NLM
Lasaro MO, Sbrogio-Almeida ME, Luiz WB, Nishimura L, Guth BEC, Ferreira LC de S. Combined immunization prtocol based on priming with parenteral DNA vaccine and boosting with oral attenuated Salmonella strain confers protective immunity to enterotoxigenic Escherichia coli in a murine model. 2003 ;[citado 2024 out. 01 ] -
Vancouver
Lasaro MO, Sbrogio-Almeida ME, Luiz WB, Nishimura L, Guth BEC, Ferreira LC de S. Combined immunization prtocol based on priming with parenteral DNA vaccine and boosting with oral attenuated Salmonella strain confers protective immunity to enterotoxigenic Escherichia coli in a murine model. 2003 ;[citado 2024 out. 01 ] - Avaliação das propriedades imunológicas e papel vacinal da flagelina fliCi de Salmonella enterica pv Typhimurium
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