Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains (2003)
- Authors:
- Autor USP: FERREIRA, LUIS CARLOS DE SOUZA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: The genetic fusion of heterologous epitopes to flagellin expressed by attenuated Salmonella strains represents a promising alternative for the development of bivalent orally delivered recombinant vaccines. In the present work we report the differential immunogenicity of two serological distinct flagellins encoded by the fliCi and fliCd genes following oral immunization of BALB/c immunized with S. Typhimurium (SL3261) or S. Dublin (SL5928) strains. Salmonella strains able to express only one flagellar type were generated following inactivation of the native chromosomal genes and transformation of the mutant strains with plasmids encoding FliCi or FliCd flagellins. The flagellins expressed by each Salmonella strains were in vitro characterized by motility tests and Western blots. Analysis of the FliCi and FliCd immunogenicity were determined in ELISA carried out with sera and fecal extracts of mice orally immunized with the recombinant strains. The results demonstrated that the Salmonella strains were able to express the recombinant, homologous or heterologous, flagellins during in vitro growth but at variable level when compared to the respective parental strains. Moreover, immunization experiments revealed that only the S. Typhimurium strain able to express the FliCi flagellin was able to elicit systemic and secreted anti-flagellin antibody responses following oral delivered of live bacterial cells. The present results suggest that phase I Salmonella flagellinspossess distinct immunological properties in mice following oral administration of attenuated strains. Such observations contribute to the understanding of the remarkable immunological properties of bacterial flagellins and may help to design new bivalent vaccines based on the expression of recombinant flagellins by attenuated Salmonella strains
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference titles: Congresso Brasileiro de Microbiologia
-
ABNT
MASSIS, L. M. e SBROGIO-ALMEIDA, M. E. e FERREIRA, Luís Carlos de Souza. Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains. 2003, Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. . Acesso em: 17 abr. 2024. -
APA
Massis, L. M., Sbrogio-Almeida, M. E., & Ferreira, L. C. de S. (2003). Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains. In . Sociedade Brasileira de Microbiologia. -
NLM
Massis LM, Sbrogio-Almeida ME, Ferreira LC de S. Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains. 2003 ;[citado 2024 abr. 17 ] -
Vancouver
Massis LM, Sbrogio-Almeida ME, Ferreira LC de S. Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains. 2003 ;[citado 2024 abr. 17 ] - Avaliação das propriedades imunológicas e papel vacinal da flagelina fliCi de Salmonella enterica pv Typhimurium
- Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines
- Evaluation of the genetic diversity among enterotoxigenic Escherichia coli (ETEC) strains isolated from humans in Brazil and Argentina
- Construção de uma vacina de DNA bivalente contra os vírus do papiloma e herpes simplex humano
- A DNA vaccine encoding the enterohemorragic Escherichia coli shiga-like toxin 2 'A ind.2' and B subunits confers protective immunity to shiga toxin challenge in the murine model
- Produção e purificação da flagelina FliCi de S. Typhimurium
- Surface expression of adhesins enhances the vaccine potential of orally delivered Bacillus subtilis strains
- Immune responses and therapeutic antitumor effects of an experimental DNA vaccine encoding human Papillomavirus type 16 oncoproteins genetically fused to Herpesvirus glycoprotein D
- Characterization of a DNA vaccine encoding the HPV-16 E7 oncoprotein genetically fused to the N-terminal domain of Salmonella enterica SV Müenchen FLICd flagellin
- Biotecnologia aplicada ao desenvolvimento de vacinas
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
