XPD transduction in human cells deficient in DNA repair by recombinant adenovirus (2003)
- Authors:
- Autor USP: MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Português
- Abstract: NER (Nucleotide Excision Repair) is the most flexible of all known DNA repair mechanisms. Two modes of NER can be distinguished: Global Genome Repair (GGR) and Transcription-coupled Repair (TCR). XPD is one of the proteins involved in GGR and TCR. It is a subunit of TFIIH complex and exhibits helicase function. Different mutations in XPD can cause different human genetic disorders as Xeroderma Pigmentosum group-D (XP-D), XP combined with Cockayne Syndrome (XPD/CS) and Trichothiodystrofy (TTD). For complementation studies of these cells, we have constructed a recombinant adenovirus (AdSHIRES-XPD), deleted in E1 and E3 regions, carrying the cDNA of XPD and the EGFP reporter gene linked by an IRES sequence (Internal Ribosome Entry Site). This construction permits both the gene of interest (cloned into the MCS) and the EGFP gene to be translated from a single bicistronic mRNA. The characterized virus was used for infection in XPD and TTD SV40-transformed fibroblasts , obtained from patients biopsies.On the basis of previous work, it was expected that this expression cassette would totally complement the XPD deficiency primary fibroblast cells. After the infection in these cells with AdSHIRES-XPD, the XPD protein was detected seventy two hours later by western-blot analysis. The virus efficiency will be evaluated by cell survival and recovery by UDS activity. We expect that this recombinant virus could open new avenues for the better understandingof cellular UV responses and cancer development
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
ARMELINI, Melissa Gava et al. XPD transduction in human cells deficient in DNA repair by recombinant adenovirus. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 30 set. 2024. -
APA
Armelini, M. G., Menck, C. F. M., Muotri, A. R., & Marchetto, M. C. N. (2003). XPD transduction in human cells deficient in DNA repair by recombinant adenovirus. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Armelini MG, Menck CFM, Muotri AR, Marchetto MCN. XPD transduction in human cells deficient in DNA repair by recombinant adenovirus. Resumos. 2003 ;[citado 2024 set. 30 ] -
Vancouver
Armelini MG, Menck CFM, Muotri AR, Marchetto MCN. XPD transduction in human cells deficient in DNA repair by recombinant adenovirus. Resumos. 2003 ;[citado 2024 set. 30 ] - Functional lentiviral vectors for xeroderma pigmentosum gene therapy
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