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Comparative study of leishmania amazonensis in mice infected by different route (2003)

• Authors:
  • Felizardo, Tânia Cristina
  • Lima, Glória Maria Collet de Araujo
• Autor USP: LIMA, GLORIA MARIA COLLET DE ARAUJO - ICB
• Unidade: ICB
• Assunto: IMUNOLOGIA
• Language: Português
• Abstract: The factors that determine the severity of cutaneous leishmaniasis have been under intense investigation. We are investigating how infection at different inoculation sites of the mouse would affect lesion development and the immune response to the parasite. We chose intradermal inoculation in the footpad or in the dorsal skin at the base of the tail. Infection was performed with 10 million L.amazonensis (Josefa strain) stationary phase promastigotes in susceptible BALB/c or resistant C57BL/6 mice and in their respective IL-4 deficient (IL-4 KO) strains. Our results show that the lesions of wild type mice (WT) of either strain were more severe when infection was performed in the dorsum than in the footpad and characteristically the former ulcerated. The lesions developed by IL-4 KO mice from both strains were smaller and no lesion was observed in C57BL/6 IL-4 KO inoculated in the dorsum. A marked inflammatory infiltrate associated with amastigote nests was present in the paw and in the dorsal lesion obtained from infected WT BALB/c or WT C57BL/6 mice.In contrast, sections from IL-4 KO mice had only few, circumscribed parasite nests. The number of parasites quantified in DLN by LDA did not vary significantly between WT or IL-4 KO BALB/c or WT C57BL/6; however IL-4 KO C57BL/6 mice inoculated in the dorsum had markedly fewer parasites in the inguinal DLN in comparison to the other groups. The antibody response and isotype distribution was notdifferent between mice inoculated in the dorsum or in the footpad. Serum levels of parasite-specific IgG1 and total IgE were higher in BALB/c than in C57BL/6 mice. Preliminary data suggest that IL-4 production by DLN cells is higher in animals infected in the footpad as compared to those inoculated in the dorsum. Taken together, our data indicate that the route of inoculation has a role in determining the outcome of the infection or lesion both in resistant and susceptible mice
• Imprenta:
  • Publisher: Comissão de Pesquisa do ICB/USP
  • Publisher place: São Paulo
  • Date published: 2003
• Source:
  • Título: Resumos
• Conference titles: Congresso Instituto Ciências Biomédicas, IV

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How to cite

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• ABNT

  FELIZARDO, Tânia Cristina e LIMA, Glória Maria Collet de Araujo. Comparative study of leishmania amazonensis in mice infected by different route. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 25 abr. 2025.

• APA

  Felizardo, T. C., & Lima, G. M. C. de A. (2003). Comparative study of leishmania amazonensis in mice infected by different route. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP.

• NLM

  Felizardo TC, Lima GMC de A. Comparative study of leishmania amazonensis in mice infected by different route. Resumos. 2003 ;[citado 2025 abr. 25 ]

• Vancouver

  Felizardo TC, Lima GMC de A. Comparative study of leishmania amazonensis in mice infected by different route. Resumos. 2003 ;[citado 2025 abr. 25 ]

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