Particular transcription patterns in a central var gene cluster in cytoadherence-selected plasmodium falciparum (2003)
- Authors:
- Autor USP: WUNDERLICH, GERHARD - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Português
- Abstract: The Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1), encoded by the multigene family named var, is responsible for the cytoadherence of infected erythrocytes in malarial infections. Approximately 50 var genes exist per parasite genome, which are mostly located in subtelomeric regions of all chromosomes, but are also found as clusters in central chromosomal regions. It was shown that almost all var transcripts are detectable in ring stage whereas in trophozoite stage one or only a few genes are transcribed while the rest of the family remains transcriptionally downregulated. Recent data published by Deitsch et al. indicate that var gene silencing requires the presence of a var intron and elements within it and an upstream element in the promoter. In the present study we selected a parasite adhesion phenotype by multiple panning procedures on E-selectin and identified a transcribed var gene in a centromeric/central cluster of 4 var genes and 1 rif gene.In order to describe factors, which were important for var transcription of one var gene or silencing of an adjacent one in the cluster, we compared the promoter and intron sequences and the var gene sequences itself, and tested the presence of transcripts. Interestingly, an adjacent 5' localized var gene containing exactly the same promoter sequence, but a different intron, appeared not to be transcribed. The same was true for a copy of another var gene localized in3´-postion of the transcribed var gene, which contained the same intron, but a different promoter compared to the transcribed var gene. All promoters localized in the cluster were of the previously described 5B1-type. Our data indicate that the combination of var promoters and var introns are critical for silencing and transcription of the respective var genes
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
GÖLNITZ, Uta; WUNDERLICH, Gerhard; TUMILASCI, V. F. Particular transcription patterns in a central var gene cluster in cytoadherence-selected plasmodium falciparum. Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. -
APA
Gölnitz, U., Wunderlich, G., & Tumilasci, V. F. (2003). Particular transcription patterns in a central var gene cluster in cytoadherence-selected plasmodium falciparum. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Gölnitz U, Wunderlich G, Tumilasci VF. Particular transcription patterns in a central var gene cluster in cytoadherence-selected plasmodium falciparum. Resumos. 2003 ; -
Vancouver
Gölnitz U, Wunderlich G, Tumilasci VF. Particular transcription patterns in a central var gene cluster in cytoadherence-selected plasmodium falciparum. Resumos. 2003 ; - Ethanolic extract of the fungus Trichoderma stromaticum decreases inflammation and ameliorates experimental cerebral malaria in C57BL/6 mice
- Determining the var gene repertoire size in Amazonian plasmodium falciparum isolates
- Dynamic activation and repression of the Plasmodium falciparum rif gene family and their relation to chromatin modification
- Rápida mudança de transcritos VAR durante infecções naturais com P. falciparum
- Symptomatic and non-symptomatic malaria infectious are caused by the same set of Plasmodium Falciparum genotypes
- Plasmodium falciparum var gene silencing / activation does not depend on the absolute promoter / intron sequence
- The South American Plasmodium falciparum var gene repertoire is limited, highly shared and possibly lacks several antigenic types
- Independent regulation of Plasmodium falciparum rif gene promoters
- Antibody recognition of Plasmodium falciparum infected red blood cells by symptomatic and asymptomatic individuals in the Brazilian Amazon
- MC.05-on cytoadhesion of Plasmodium vivax - infected erithrocytes
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