Effect of thyroid hormone on angiotensin type 1 receptor (at1) expression in cultured cardiac myocytes and fibroblasts (2003)
- Authors:
- Autor USP: CHAVES, MARIA LUIZA MORAIS BARRETO DE - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Português
- Abstract: Objetivo: Renin-Angiotensin System (RAS) has been associated with cardiac hypertrophy induced by thyroid hormone. However, little is known regarding the regulation of AT1 receptor in cardiac cells after hyperthyroidism. Métodos e Resultados: Newborn rat ventricular cardiomyocytes and fibroblasts were obtained from 1 to 2 day old Wistar rats. After successive digestion of the tissue, cardiomyocytes and fibroblasts were separated using a Percoll gradient and pooled in DMEN enriched with Horse or Foetal Bovine Serum, respectively. 24 hours before the treatment, cells were maintained in serum free media. Cells were treated with crescent doses of T3 (1?M - 1µM) during 24 hours. After that total RNA was extracted from two cell types and AT1 expression analyzed using semiquantitative RT-PCR. Results: After treatment, cardiomyocytes showed a significative decrease on expression level of AT1, characterizing a dose-response relation, which showed the lowest expression level with 1 µM of T3.) On the other hand, the treatment with T3 in cardiac fibroblasts did not significantly change the expression level of AT1, independent of dose used. Conclusões: The effect of Thyroid hormone on decreasing AT1 receptors levels on cardiac myocytes may play an important regulatory role considering that both Angiotensin II and Thyroid Hormone induced cardiac hypertrophy
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Curitiba, Paraná
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
CARNEIRO RAMOS, M. S.; SILVA, V. B.; BARRETO-CHAVES, Maria Luiza Morais. Effect of thyroid hormone on angiotensin type 1 receptor (at1) expression in cultured cardiac myocytes and fibroblasts. Anais.. Curitiba, Paraná: Federação de Sociedades de Biologia Experimental, 2003. -
APA
Carneiro Ramos, M. S., Silva, V. B., & Barreto-Chaves, M. L. M. (2003). Effect of thyroid hormone on angiotensin type 1 receptor (at1) expression in cultured cardiac myocytes and fibroblasts. In Resumos. Curitiba, Paraná: Federação de Sociedades de Biologia Experimental. -
NLM
Carneiro Ramos MS, Silva VB, Barreto-Chaves MLM. Effect of thyroid hormone on angiotensin type 1 receptor (at1) expression in cultured cardiac myocytes and fibroblasts. Resumos. 2003 ; -
Vancouver
Carneiro Ramos MS, Silva VB, Barreto-Chaves MLM. Effect of thyroid hormone on angiotensin type 1 receptor (at1) expression in cultured cardiac myocytes and fibroblasts. Resumos. 2003 ; - Modulation of the ubiquitin proteasome system in the thyroid hormone-induced cardiac hypertrophy
- Modulação da via da AMPK pelo hormônio tiroideano em culturas de cardiomiócitos
- Reactive oxygen and nitrogen species balance in the determination of thyroid hormones-induced cardiac hypertrophy mediated by renin–angiotensin system
- Hipertireoidismo afeta a hidrólise de nucleotídios da adenina em medula espinhal de ratos em diferentes fases do desenvolvimento
- Hipertireoidismo modifica as atividades ectonucleotidásicas de sinaptosoma de hipocampo e córtex cerebral de ratos em diferentes fases do desenvolvimento
- Effects of chronic treatment with L-thyroxin on learning and memory: a behavioral approach to animal models of hyperthyroidism
- Efeito o hormônio tireideano sobre as ectonucleotidases em culturas primárias de fibroblastos cardíacos de ratos adultos
- The hyperthyroidism and the renin-angiotensin system on cardiac microcirculation
- Hipotireoidismo afeta a resposta nociceptiva e a hidrólise de nucleotídios da adenina em sinaptossoma de medula espinhal de ratos em diferentes fases do desenvolvimento
- 5'-Nucleotidase activity is altered by hypo-and hyperthyroidism in platelets from adult rats
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
