Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid (2002)
- Authors:
- USP affiliated authors: GRANJEIRO, JOSE MAURO - FOB ; TAGA, EULAZIO MIKIO - FOB
- Unidade: FOB
- Subjects: PROTEÍNAS; ÁCIDOS; INIBIÇÃO
- Language: Inglês
- Abstract: Uric acid inhibited 50% of the activity of bovine kidney low molecular mass phosphotyrosine protein phosphatase at concentrations of 1.0, 0,4, 1,3, and 0,2 mM, respectively for p-nitrophenyl phosphate (p -NPP), flavine mononucleotide, 'beta'-naphthyl phosphate and tyrosine phosphate (Tyr-P) as substrates. The mixed type inhibition of p-NPP hydrolysis was fully reversible, with 'K IND. ic' and 'K IND. iu' values of 0.4 and 1.0 mM, respectively; the inhibition by uric acid shifted the pH optimum from 5.0 to 6.5. Whem Tyr-P was the substrate, competitive inhibition was observed with a 'K IND. i' value of 0.05 mM. Inhibition studies by uric acid in the presence of thiol compounds, and preincubation studies in the presence of inorganic phosphated suggest that the interaction of uric acid with the enzyme occured at the active site, but did not involve SH residues, and that the mechanism of inhibition depended on the structure of the substrates
- Imprenta:
- Source:
- Título: Journal of Enzyme Inhibition and Medicinal Chemistry
- ISSN: 8755-5093
- Volume/Número/Paginação/Ano: v. 17, n. 5, p. 345-350, 2002
-
ABNT
GRANJEIRO, José Mauro et al. Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid. Journal of Enzyme Inhibition and Medicinal Chemistry, v. 17, n. 5, p. 345-350, 2002Tradução . . Acesso em: 11 fev. 2026. -
APA
Granjeiro, J. M., Ferreira, C. V., Granjeiro, P. A., Silva, C. C. da S., Volpe, P. L. O., & Aoyama, H. (2002). Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid. Journal of Enzyme Inhibition and Medicinal Chemistry, 17( 5), 345-350. -
NLM
Granjeiro JM, Ferreira CV, Granjeiro PA, Silva CC da S, Volpe PLO, Aoyama H. Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid. Journal of Enzyme Inhibition and Medicinal Chemistry. 2002 ; 17( 5): 345-350.[citado 2026 fev. 11 ] -
Vancouver
Granjeiro JM, Ferreira CV, Granjeiro PA, Silva CC da S, Volpe PLO, Aoyama H. Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid. Journal of Enzyme Inhibition and Medicinal Chemistry. 2002 ; 17( 5): 345-350.[citado 2026 fev. 11 ] - Caracterização ultraestrutural e análise morfométrica da resposta celular ao parafuso de osso bovino misto em subcutâneo de ratos
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