Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil (2002)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; MUNFORD, VERIDIANA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Infantile diarrhea is responsible for many pediatric hospitalizations and for high mortality in developing countries. Rotavirus is the main pathogen associated with diarrhea in young children. Fecal samples (236) were collected from December/1995 to November/1999 from 3 hospitals and one ambulatory in São Paulo city, Brazil. Samples were submitted to the enzyme immunoassay for rotavirus and adenovirus (EIARA-FIOCRUZ) and polyacrylamide gel eletrophoresis (PAGE). Results showed 44 (18,6%) samples positive for rotavirus by EIARA and 35(14,8%) by PAGE. Samples were then analyzed by genotyping RT-PCR. Two sets of primers were used, one for G genotypes (G1, G2, G3, G4 and G9) and other for P genotypes (P[8], P[9], P[4], P[10] and P[6]). Preliminary results for G genotype characterization showed 6 G1 samples, 5 G2, 2 G4 and 5 G9 samples. Five samples showed mixed genotypes: one sample was characterized as G1+G4+G9, 2 as G1+G4 and 2 as G1+G2+G4. For P genotype, results are: 5 P[4] samples, 7 P[8] and 4 samples were characterized as mixtures of P[4]+P[8] genotypes. These results confirms the emergence of G9 genotype, detected in São Paulo since 1996 and shows the value of the study of rotavirus genotypes, with important applications for vaccine strategies
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
SCHMICH, H et al. Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 29 dez. 2025. -
APA
Schmich, H., Fagundes Neto, U., Munford, V., & Racz, M. L. B. de O. (2002). Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Schmich H, Fagundes Neto U, Munford V, Racz MLB de O. Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil. Resumos. 2002 ;[citado 2025 dez. 29 ] -
Vancouver
Schmich H, Fagundes Neto U, Munford V, Racz MLB de O. Detection and molecular caracterization of G and P genotypes of human rotavirus from São Paulo, Brazil. Resumos. 2002 ;[citado 2025 dez. 29 ] - Electrophoretic and serological characterization of bovine rotavirus samples from an outbreak of diarrhea in a dairy farm in state of goias, brazil
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- Serological and electrophoretic characterization of bovine rotavirus identified in dairy herds from district of piracanjuba, goias
- Epidemiological typing of rotavirus using rna-dna hybridization
- Detection of bovine rotavirus G6P[11] in an outbreak of diarrhea in dairy herds with high frequence of mixed rotavirus infection
- Ocorrencia de rotavirus em bezerros neonatos provenientes de rebanhos leiteiros do municipio de piracanjuba, goias
- Serological and genotypical characterization of group a rotavirus samples from children of Goiania-Goias, Brazil
- Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil
- Molecular characterization of astrovirus in stool samples from children in São Paulo, Brazil
- Sorotipagem e caracterização molecular de genotipos G e P de amostras de rotavírus suínos
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