Over expression of DNA repair proteins XPA and XPC in primary normal and deficient cells andt its relation with apoptosis (2002)
- Autor:
- Autor USP: MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Nucleotide excision repair (NER) is one of the most versatile repair mechanisms that ensures proper functioning and faithful transmission of genetic information in all living cells. The phenotypes consequent upon NER defect in humans are autosomal recessive diseases like xeroderma pigmentosum (XP). XP individuals from group A are defective in the XPA protein essential for NER and serve, together with other NER proteins, as a nucleation factor for DNA damage recognition in both transcribed and non-transcribed genome areas. The XPC encoded protein acts directly in the global genome repair (non-transcribed regions and non-transcribed strands from active genes). This protein acts during the recognition of DNA damage induced by ultraviolet light (UV) at the beginning of nucleotide excision repair process. In this work, we intend to search for the causes of cell death after UV damage in XP cells corrected or not by the complementing recombinant adenovirusThe analysis was performed by the detection of apoptosis after UVB (290-320 nm) irradiation. The results indicate that UV-B irradiated cells infected with recombinant XPA/C adenovirus die less through apoptosis than non-infected cells. These data suggest that the recovery of the DNA repair ability in cells, containing over expressed DNA repair genes after adenovirus infection, is involved with a blockage of apoptosis in deficient cells. However, normal cells infected with recombinant XPA/C adenovirus showed recovery ofapoptosis when irradiated and this could be an effect of the adenovirus machinery background. This should be further investigated. We believe that the efficient delivery machinery of adenovirus vectors would be an important strategy for better understanding the phenotypic correction by gene transfer in XP fibroblasts
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
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ABNT
MENCK, Carlos Frederico Martins. Over expression of DNA repair proteins XPA and XPC in primary normal and deficient cells andt its relation with apoptosis. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 29 dez. 2025. -
APA
Menck, C. F. M. (2002). Over expression of DNA repair proteins XPA and XPC in primary normal and deficient cells andt its relation with apoptosis. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Menck CFM. Over expression of DNA repair proteins XPA and XPC in primary normal and deficient cells andt its relation with apoptosis. Resumos. 2002 ;[citado 2025 dez. 29 ] -
Vancouver
Menck CFM. Over expression of DNA repair proteins XPA and XPC in primary normal and deficient cells andt its relation with apoptosis. Resumos. 2002 ;[citado 2025 dez. 29 ] - DNA Polimerase in sugar cane Xylella fastidiosa and Xanthomonas axonopodis pv. citri
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- Complementation of xeroderma pigmentosum variant cells by a recombinant adenovirus carrying the polymerase ETA cDNA
- Análise funcional do gene lexA de Caulobacter crescentus e sua implicação para a resposta SOS
- Death pathways and P53 response after introduction of different DNA lesions on the double-helix
- Alternative response pathways to ultraviolet irradiation in the lack of nucleotide excision repair
- Hepatitis B virus surface antigen (HBsAg) expression by viral vectors in vero cells
- Complete recovery of he DNA repair deficiency in human Xeroderma pigmentosum group a cells by recombinant adenovirus infection
- Putative new organellar DNA polymerases in plants
- DNA polimerases em cana-de-açucar
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