Plasma autoantibody reactivy in hypercholesterolemic (HC) against oxidatively modified low density loprotein (MOLDL) is different from that of normocholesterolemic (NC) (2002)
- Authors:
- Autor USP: GIDLUND, MAGNUS AKE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Objetivo: Atherosclerosis is a chronic inflammatory process in which components of the immune system (i.e., antibodies and T-cells) play a pivotal role. As moLDL has been implicaded in the atherosclerotic process the aim of this work was to test chromatographically separated (Size exclusion) subfractions of moLDL (oxidized with copper ) for their reactivity to autoantibodies in plasma of HC that is known to have a higher risk for coronary heart diseases (CHD) and compare the pattern of reactivity to that of plasma autoantibodies found in NC. Métodos e Resultados: moLDL obtained by oxidation of LDL (20 uM CuSO4, in vitro) was subfractionated using FPLC and Superdex 200 column (Pharmacia, Sweden) and a diode-array detector (Shimadzu, Japan). The total oxLDL and the protein peak fractions were assayed by ELISA for their reactivity with sera from HC (n=30, cholesterol > 250 mg/dL) and NC (n=30, cholesterol < 200 mg/dL). We could obtained 4 principal new peaks from moLDL. Of these peaks ( low molecular weight) one showed different reactivity with HC plasma than with NC. Conclusões: We can conclude that HC is a condition where we can detect a different spectrum of autoantibody reactivity against moLDL compared to NC. We think that this approach can be a useful methodology in the search for specific antigens in modified LDL involved in the formation of auto-antibodies in patients that are prone to develop atherosclerosis
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
KETELHUTH, D F J; FREITAS, F C S; CARVALHO, M D T; BOSCHCOV, P. Plasma autoantibody reactivy in hypercholesterolemic (HC) against oxidatively modified low density loprotein (MOLDL) is different from that of normocholesterolemic (NC). Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. -
APA
Ketelhuth, D. F. J., Freitas, F. C. S., Carvalho, M. D. T., & Boschcov, P. (2002). Plasma autoantibody reactivy in hypercholesterolemic (HC) against oxidatively modified low density loprotein (MOLDL) is different from that of normocholesterolemic (NC). In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Ketelhuth DFJ, Freitas FCS, Carvalho MDT, Boschcov P. Plasma autoantibody reactivy in hypercholesterolemic (HC) against oxidatively modified low density loprotein (MOLDL) is different from that of normocholesterolemic (NC). Resumos. 2002 ; -
Vancouver
Ketelhuth DFJ, Freitas FCS, Carvalho MDT, Boschcov P. Plasma autoantibody reactivy in hypercholesterolemic (HC) against oxidatively modified low density loprotein (MOLDL) is different from that of normocholesterolemic (NC). Resumos. 2002 ; - Opposing effects between short-term combined transdermal estradiol/noretisterone against oxidized LDL (oxLDL) and heat shock proteins (hsp)
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