Genotype characterization of group a rotavirus from São Paulo city, SP, Brazil (2001)
- Authors:
- USP affiliated authors: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB ; DURIGON, EDISON LUIZ - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Human rotavirus is an important agent of acute gastroenteritis of young children. Two viral proteins of the outer capsid of rotavirus are independently involved in virus neutralization: VP7, defining G serotypes and VP4, defining P serotypes/genotypes. We studied 158 stool samples from children with ages between 0 and 5 years, in São Paulo city, Brazil. Samples were collected from March/94 to September/95, in the ambulatory of University of São Paulo Hospital. Rotavirus and adenovirus were identified in stools by a combined enzyme immunoassay (EIARA-FIOCRUZ). Fifty five samples (34,8%) were positive for rotavirus and 5 (3,2%) were positive for adenovirus in EIARA test. Objectives: The objective of this study was to characterize that samples by RT-PCR. Methods e Results: Rotavirus positive samples were submitted to G and P genotyping by extraction of dsRNA and reverse transcription followed by polymerase chain reaction (RT-PCR), with set of primers specific for human genotypes. Negative samples were submitted to RT-PCR with set of primers specific for bovine and swine rotaviruses. G genotyping results showed 26 (47,3%) samples with G[2] specificity and 6 (10,9%) G[1] samples. Genotypes G[3],G[4] and G[5] were found in one sample each. Moreover, 10 (18,1%) samples showed a mixture of genotypes: G[4][9],G[1][5], G[2][4][9], G[2][4] and G[1][2][3]. P genotype results were: 18 (32,7%) P[4] samples, 16 (29,1%) P[8] and 5 (9,1%) P[6] samples. Mixture ofgenotypes P[4][8] and P[4][6][8] was found in 10% of the samples and 1 sample was characterized as P[4][6]. Unusual combinations of G[2] with P genotypes were found in 21,8% of samples: 5 (9,1%) samples with G[2]P[6], 4 (7,3%) with G[2]P[8] and 3 (5,5%) with mixture of two or three P genotypes. conclusions: The results show the diversity of group A human rotavirus in São Paulo, especially in samples with G[2] specificity, found in high proportion of positive samples Human rotavirus is an important agent of acute gastroenteritis of young children. Two viral proteins of the outer capsid of rotavirus are independently involved in virus neutralization: VP7, defining G serotypes and VP4, defining P serotypes/genotypes. We studied 158 stool samples from children with ages between 0 and 5 years, in São Paulo city, Brazil. Samples were collected from March/94 to September/95, in the ambulatory of University of São Paulo Hospital. Rotavirus and adenovirus were identified in stools by a combined enzyme immunoassay (EIARA-FIOCRUZ). Fifty five samples (34,8%) were positive for rotavirus and 5 (3,2%) were positive for adenovirus in EIARA test. Objectives: The objective of this study was to characterize that samples by RT-PCR. Methods e Results: Rotavirus positive samples were submitted to G and P genotyping by extraction of dsRNA and reverse transcription followed by polymerase chain reaction (RT-PCR), with set of primers specific for human genotypes. Negative samples weresubmitted to RT-PCR with set of primers specific for bovine and swine rotaviruses. G genotyping results showed 26 (47,3%) samples with G[2] specificity and 6 (10,9%) G[1] samples. Genotypes G[3],G[4] and G[5] were found in one sample each. Moreover, 10 (18,1%) samples showed a mixture of genotypes: G[4][9],G[1][5], G[2][4][9], G[2][4] and G[1][2][3]. P genotype results were: 18 (32,7%) P[4] samples, 16 (29,1%) P[8] and 5 (9,1%) P[6] samples. Mixture of genotypes P[4][8] and P[4][6][8] was found in 10% of the samples and 1 sample was characterized as P[4][6]. Unusual combinations of G[2] with P genotypes were found in 21,8% of samples: 5 (9,1%) samples with G[2]P[6], 4 (7,3%) with G[2]P[8] and 3 (5,5%) with mixture of two or three P genotypes. conclusions: The results show the diversity of group A human rotavirus in São Paulo, especially in samples with G[2] specificity, found in high proportion of positive samples
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
KROEFF, S S et al. Genotype characterization of group a rotavirus from São Paulo city, SP, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 19 abr. 2024. -
APA
Kroeff, S. S., Rácz, M. L., Taddei, C. R., Souza, E. C., Durigon, E. L., & Munford, V. (2001). Genotype characterization of group a rotavirus from São Paulo city, SP, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Kroeff SS, Rácz ML, Taddei CR, Souza EC, Durigon EL, Munford V. Genotype characterization of group a rotavirus from São Paulo city, SP, Brazil. Resumos. 2001 ;[citado 2024 abr. 19 ] -
Vancouver
Kroeff SS, Rácz ML, Taddei CR, Souza EC, Durigon EL, Munford V. Genotype characterization of group a rotavirus from São Paulo city, SP, Brazil. Resumos. 2001 ;[citado 2024 abr. 19 ] - Analysis of VP4 sequence of an unusual porcine rotavirus strain from southern Brazil
- Genotype characterization of group a rotavirus from São Paulo City, SP, Brazil
- Sequence analysis of atypical G genotype in bovine rotavirus samples from state of Goias, Brazil
- Diversity of G2 serotypes/genotypesof human rotavirus in São Paulo, Brazil
- Molecular characterização by gene sequence of atypical genotype G[3] bovine rotavirus sample from the state of Goiás , Brazil
- Unusual G and P genotypes of porcine rotavirus from Paraná and Santa Catarina states, Brazil
- Genotype characterization of group a rotavirus from São Paulo City, SP, Brazil
- Molecular characterization of porcine rotaviruses from southern region of brazil: Characterization of an atypical genotype G[9] strain
- Molecular characterization by gene sequence of atypical genotype G[3] bovine rotavirus sample from the state of Goiás, Brazil
- Molecular characterization of swine rotaviruses from South Region: characterization of G9 genotypes strain
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