Gestational alcohol exposure facilitates parkinson's disease in the aged offspring: efeitos sobre a atividade da proteína quinase dependente de AMPc (PKA) (2001)
- Authors:
- Autor USP: CHADI, GERSON - ICB
- Unidade: ICB
- Assunto: FARMACOLOGIA
- Language: Inglês
- Abstract: Objectives: Prenatal exposure to ethanol delays the development of dopamine (DA) system. We have employed comportamental analysis and quantitative stereology combined to tyrosine hydroxylase (TH) immunohistochemistry to evaluate the long-lasting effects of gestational alcoholism on the offspring dopamine system. Method and Results: Pregnant Wistar rats received a hyper-proteic liquid diet with 37.5% ethanol-derived calories (EDC) or with maltose-dextrin substituted for ethanol (MDC) throughout gestation. Embryos on E19 and offspring of two years of age (24M) from both diet groups were sacrificed and brains processed for TH immunohistochemistry. Also, 24M offspring was submitted to a partial nigrostriatal DA lesion by mean of a stereotaxical striatal injection of 6-hydroxydopamine (6-OHDA). Control offspring was injected with solvent. Circling behavior was tested in the second week of lesion by a subcutaneous injection of apomorphine (APO, 0.05mg/kg) and registered in an automated rotometer which monitor full (360º) body turns in either direction. APO-induced turns were recorded over successive 5-min interval every 48 hours for 8 days. Lesioned offspring was sacrificed 14 days after surgeryOptical fractionator was used to quantify immunoreactive buttons (IB) on terminals in the bilateral neostriatum (ST) and DA neuron in the entire bilateral pars compacta of the substantia nigra (SNc). The same was done in the ipsilateral nigrostriatal system in the lesionedaged offspring. The rotator was used to measure the DA cell volume and the point intercepts in the measurements of the volumeal fraction of the DA fibers in the SNc. Estimated total number and density of DA neurons, as well as the volumeal fraction of cell processes decreased by 34.7%, 20.7% and 16 %, respectively, in SNc of E19 EDC group, however, no changes in DA neuron numbers were observed in the 24M offspring. The number of DA IB was decreased (39%) in EDC embryos. Contraversive circling behavior was increased in 6-OHDA lesioned EDC. The 6-OHDA lesion promoted a larger lesion (72.3%) as well as a larger disappearance (33.5%) of DA IB in the striatum in the aged offspring of the alcohol group. Both DA neuronal number and volumeal fraction were decreased in the SNc of EDC/6-OHDA group (p<0.01 and p<0.05, respectively). The Gaussian curve of normal distribution of logarithmic plotted values of DA neuronal volumes shifted to the rigth in the lesioned alcohol exposed offspring. Conclusion: Ethanol consumption during gestation impairs the DA system development which may favor nigrostriatal DA degeneration throughout life
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
GOMIDE, V. C. e CHADI, Gerson. Gestational alcohol exposure facilitates parkinson's disease in the aged offspring: efeitos sobre a atividade da proteína quinase dependente de AMPc (PKA). 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 22 jan. 2026. -
APA
Gomide, V. C., & Chadi, G. (2001). Gestational alcohol exposure facilitates parkinson's disease in the aged offspring: efeitos sobre a atividade da proteína quinase dependente de AMPc (PKA). In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Gomide VC, Chadi G. Gestational alcohol exposure facilitates parkinson's disease in the aged offspring: efeitos sobre a atividade da proteína quinase dependente de AMPc (PKA). Resumos. 2001 ;[citado 2026 jan. 22 ] -
Vancouver
Gomide VC, Chadi G. Gestational alcohol exposure facilitates parkinson's disease in the aged offspring: efeitos sobre a atividade da proteína quinase dependente de AMPc (PKA). Resumos. 2001 ;[citado 2026 jan. 22 ] - On the presence of the bfgf immunoreactivity in the spinal cord and dorsal root ganglia of the adult rat
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