Detection of hepatitis a in waste water samples in São Paulo city, Brazil (2001)
- Authors:
- USP affiliated authors: MEHNERT, DOLORES URSULA - ICB ; HARSI, CHARLOTTE MARIANNA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Human enteric viruses were detected in sewage, surface and underground water worldwide. However, to estimate the risk of this findings for the public health many informations about the types of viruses and numbers of infection units, which are circulating, and seasonal occurrence need to be known. HAV outbreaks associated to the comsuption of contaminated water occur, but the numbers are underestimate in most countries. The aim of the present study was to report the occurrence of hepatitis A virus (HAV) in sewage and surface water in São Paulo city. A total of 43 samples of raw sewage and sewage-polluted creek water were collected at two different sites in city of São Paulo through July 1998 to June 1999: Sewage Pumping Station Edu Chaves and Creek Pirajussara, located onorth and south districts of the city, respectively. Four-liter samples were submitted to a two-step concentration procedure based on filtration through a microporous electropositive charged filter membrane (Zeta Plus 60S) and ultracentrifugation. HAV were detected by RT-PCR technique using the primers HAVC-R and HAVC-L, designed by De Leon et al. (1990) for the amplification of a 192 bp fragment, corresponding to the interface of V1-VP3 genes. HAV were detected in 23 (53.5%) out of 43 examined samples by RT-PCR, followed by reamplification reaction in sewage and creek water through out the year
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
SASSAROLI, A et al. Detection of hepatitis a in waste water samples in São Paulo city, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 02 jan. 2026. -
APA
Sassaroli, A., Garrafa, P., Santos, F. M., Mehnert, D. U., Vieira, M. J., Monezi, T. A., et al. (2001). Detection of hepatitis a in waste water samples in São Paulo city, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Sassaroli A, Garrafa P, Santos FM, Mehnert DU, Vieira MJ, Monezi TA, Barardi CRM, Hársi CM. Detection of hepatitis a in waste water samples in São Paulo city, Brazil. Resumos. 2001 ;[citado 2026 jan. 02 ] -
Vancouver
Sassaroli A, Garrafa P, Santos FM, Mehnert DU, Vieira MJ, Monezi TA, Barardi CRM, Hársi CM. Detection of hepatitis a in waste water samples in São Paulo city, Brazil. Resumos. 2001 ;[citado 2026 jan. 02 ] - Studies on the HADV-41 interaction with the host-cell
- Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes
- Resistence of rotavirus adsorbed to zeta plus 60S filter membranes
- Occurrence of human enteric viruses in sewage and surface waters un the city São Paulo
- Virus a new parameter for determination of water quality
- Distribution of rotavirus G1-G5 genotypes in domestic effluent and creek in São Paulo city from 2000 to 2001
- Rotavirus concentration from water samples: re-evaluation of the efficiency of the ZP60S filter membrane
- Genome type analysis of adenovirus subgenus c isolated in Sao Paulo
- Studies on the hadv-41 interaction with the host-cell; functions of the short and long fibers at the viral cycle
- Estudo dos receptores celulares para adenovírus humano, sorotipo 41 (HADV-41)
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