Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain (2001)
- Authors:
- USP affiliated authors: MOSIG, JOSE MARIA ALVAREZ - ICB ; LIMA, MARIA REGINA D'IMPERIO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Induction of mucosal immune responses by parenterally delivered DNA vaccines are usually hard to attain. In this work we evaluated a combined vaccination protocol, based on intramuscularly (i.m.) delivered DNA vaccine followed by oral administration of recombinant attenuated Salmonella typhimurium vaccine strain, as an approach to activate systemic and secreted antibody responses in BALB/c mice against the CFA/I fimbriae of enteroxigenic Escherechia coli (ETEC), a model antigen employed in our immunization experiments. Mice were i.m. immunized twice, two weeks apart, with 100'quadrado'g doses of pRECFA, a plasmid vector encoding the CFA/I anigen, followed two weeks later by two oral doses('10POT.10'c.f.u.), given two weeks apart, of HG3, a CFA/I-expressing derivative of the aroA S. typhimurium strain SL3261. The results showed that CFA/I-specific serum IgG mucosal Iga were synergistically enhanced in mice of different ages submitted to the combined vaccination protocol. Similar results were found using the number of CFA/I-specific antibody secreting cells in spleens, mesenteric lymph nodes and Peyer's patches of vaccinated mice. Additional experiments have shown that inactivated HG3 as well as CFA/I-producing ETEC strains can also be used as oral boosting antigens. The strategy induced also a memory response detected up to one year following the DNA vaccination. These results suggest that parenterally administered DNA vaccine can primelocal and secreted immune responses in mice as demonstrated in mice immunized with oral particulate vaccines and represent a promising vaccination approach against enteric pathogens
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
LÁSARO, M O et al. Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 03 nov. 2024. -
APA
Lásaro, M. O., Sbrogio-Almeida, M. E., Sardinha, L. R., Bastos, K., Alvarez, J. M., & Lima, M. R. D. 'I. (2001). Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Lásaro MO, Sbrogio-Almeida ME, Sardinha LR, Bastos K, Alvarez JM, Lima MRD'I. Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain. Resumos. 2001 ;[citado 2024 nov. 03 ] -
Vancouver
Lásaro MO, Sbrogio-Almeida ME, Sardinha LR, Bastos K, Alvarez JM, Lima MRD'I. Enhanced systemic and secreted antibody responses in mice submitted to a combined immunization protocol based on parental (priming) DNA vaccine and oral (boosting) delivered salmonella vaccine strain. Resumos. 2001 ;[citado 2024 nov. 03 ] - IFN-γ priming effects on the maintenance of effector memory CD4(+) T cells and on phagocyte function: evidences from infectious diseases
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