Trabalho de evento-resumo

Flip and Flop isoforms of GlutR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions (2000)

• Authors:
  • Pires, R. S.
  • Rebouças, Nancy Amaral
  • Duvoisin, R. M.
  • Britto, Luiz Roberto Giorgetti de
• USP affiliated authors: REBOUCAS, NANCY AMARAL - ICB ; BRITTO, LUIZ ROBERTO GIORGETTI DE - ICB
• Unidade: ICB
• Assunto: FISIOLOGIA
• Language: Inglês
• Abstract: Objetivo: Glutamate is the main excitatory transmitter in the retinotectal system. Molecular cloning of glutamate receptor (GluR) subunits has permitted an improved knowledge of GluR localization in the nervous system. The aim of this study was to investigate the expression patterns of the flip and flop isoforms of GluR1 mRNA in the chick optic tectum and also analyse possible changes in their expression in response to retinal lesions. Métodos e Resultados: Chicks were anesthetized with ketamine and xylazine and submitted to unilateral retinal lesions. After survival times of 2 and 7 days, the animals were sacrificed and both the control and deafferented tecta were removed for total RNA extraction. A sensitive RNase protection assay was employed to determine mRNA levels of flip and flop splicing variants of the GluR1 subunit. The distribution profile of specific mRNAs showed that the flop isoform is more strongly expressed in the tectum than the flip variant.Retinal lesions produced marked effects upon mRNA levels of both flip and flop variants of GluR1. Two days following the lesion, the mRNA levels of GluR1 flip and flop variants decreased in the deafferented tectum in relation to the ipsilateral (control) tectum. After 7 days, the mRNA levels of both GluR1 isoforms were increased. However, the flip isoform mRNA showed a more marked increase than the flop mRNA. In contrast, 18S ribosomal RNA levels, measured inparallel as a control, were unchanged. Conclusões: These results indicate that retinal lesions may induce a down-regulation of flip and flop variants of GluR1 over the first 2 days, followed by an up-regulation of both isoforms after 7 days. These effects could be due to the removal of the glutamatergic input from retinal ganglion cells and/or the withdrawal of a retinotectal trophic factor
• Imprenta:
  • Publisher: FESBE
  • Publisher place: São Paulo
  • Date published: 2000
• Source:
  • Título: Resumos
• Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental

---

How to cite

A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas

• ABNT

  PIRES, R. S. et al. Flip and Flop isoforms of GlutR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions. 2000, Anais.. São Paulo: FESBE, 2000. . Acesso em: 09 abr. 2026.

• APA

  Pires, R. S., Rebouças, N. A., Duvoisin, R. M., & Britto, L. R. G. de. (2000). Flip and Flop isoforms of GlutR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions. In Resumos. São Paulo: FESBE.

• NLM

  Pires RS, Rebouças NA, Duvoisin RM, Britto LRG de. Flip and Flop isoforms of GlutR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions. Resumos. 2000 ;[citado 2026 abr. 09 ]

• Vancouver

  Pires RS, Rebouças NA, Duvoisin RM, Britto LRG de. Flip and Flop isoforms of GlutR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions. Resumos. 2000 ;[citado 2026 abr. 09 ]

Últimas obras dos mesmos autores vinculados com a USP cadastradas na BDPI:

• Expression pf endo-oligopeptidase Ain the rat central mervous system: a non-radioactive in situ hybridization study
• Efeitos das lesões retinianas sobre a expressão do mRNA das isoformas flip e lop das subunidades GluR 2 e 3 dos receptores de glutamato no tecto óptico de pintos
• Upregulation of NHE3 is associated with compensatory cell growth response in young uninephrectomized rats
• Retinal lesions induce differential changes in the expression of flip and flop isoforms of the glutamate receptor subunit GluR1 in the chick opitic tectum
• Flip and flop isoforms of gluR1 glutamate receptor mRNA in the chick tectum: effects of retinal lesions
• Modulation of NHE3 expression by chronic metabolic and respiratory acidosis
• Clonagem e identificacao do cdna que codifica um trocador 'NA POT.+' / 'H POT.+' De guelras posteriores de chasmagnatus granulata
• Modulacao da expressao da isoforma 3 do trocador 'NA POT.+' / 'H POT.+' (Nhe-3) em tecido renal de ratos por paratormonio
• Clonagem e identificacao do cdna que codifica o trocador 'NA POT.+' / 'H POT.+' De guelra de chasmagnathus granulata
• Glucocorticoid and Mineralocordicoid Action on Luminal Acidification of Proximal Tubules: Different Action Mechanisms or Different Na/H exchanger Target Isoform?