Effect of two bisphosphonates associated or not to Laser Phototherapy on the viability and proliferation of cultured cells (2014)
- Authors:
- USP affiliated authors: DEBONI, MARIA CRISTINA ZINDEL - FO ; HOMEM, MARIA DA GRAÇA NACLERIO - FO ; HANNA, ANDRÉIA APARECIDA TRAINA - FO
- Unidade: FO
- Subjects: LASER NÃO CIRÚRGICO; CÉLULAS CULTIVADAS
- Language: Português
- Abstract: Despite several forms of treatment of bisphosphonate (BP) related osteonecrosis of jaw (BRONJ) have been reported, no consensus protocol has been established. The sodium alendronate and zoledronic acid in different concentrations are BPs currently applied for treating BRONJ. Laser phototherapy (LPT) using red wavelength has shown positive effects on BRONJ lesions healing. This study aimed to evaluate the viability and proliferation of osteoblasts and fibroblasts submitted to sodium alendronate and zoledronic acid associated or not to LPT. Cells were cultured in DMEM containing one of the BPs in different concentrations for 24 hours. The LPT was then applied by using a continuous diode laser (InGaAlP, 660nm, 30mW, spot size of 0.028 cm2) in two different energy densities (5J/cm2 and 10J/cm2, 4,5 and 9s, respectively), on punctual and contact mode. Two irradiations with 6 hoursinterval were performed. Cell viability and proliferation were determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) reduction assay in three different periods 24h, 48h and 72h after first irradiation. Both bisphosphonates were cytotoxic to osteoblast and fibroblasts in culture in a dose-dependent manner. The amount of viable cells was similar in lased and non-lased groups
- Imprenta:
- Source:
- Título do periódico: Brazilian Oral Resarch
- ISSN: 1806-8324
- Volume/Número/Paginação/Ano: v.28, supl. 1, p. 40, res. AO015, 2014
- Conference titles: Reunião Anual SBPqO
-
ABNT
BROZOSKI, Mariana Aparecida et al. Effect of two bisphosphonates associated or not to Laser Phototherapy on the viability and proliferation of cultured cells. Brazilian Oral Resarch. São Paulo: Faculdade de Odontologia, Universidade de São Paulo. . Acesso em: 20 set. 2024. , 2014 -
APA
Brozoski, M. A., Ferreira, L. S., Moura-Netto, C., Borba, A. M., Traina, A. A., Deboni, M. C. Z., et al. (2014). Effect of two bisphosphonates associated or not to Laser Phototherapy on the viability and proliferation of cultured cells. Brazilian Oral Resarch. São Paulo: Faculdade de Odontologia, Universidade de São Paulo. -
NLM
Brozoski MA, Ferreira LS, Moura-Netto C, Borba AM, Traina AA, Deboni MCZ, Marques MM, Homem M da GN. Effect of two bisphosphonates associated or not to Laser Phototherapy on the viability and proliferation of cultured cells. Brazilian Oral Resarch. 2014 ;28 40.[citado 2024 set. 20 ] -
Vancouver
Brozoski MA, Ferreira LS, Moura-Netto C, Borba AM, Traina AA, Deboni MCZ, Marques MM, Homem M da GN. Effect of two bisphosphonates associated or not to Laser Phototherapy on the viability and proliferation of cultured cells. Brazilian Oral Resarch. 2014 ;28 40.[citado 2024 set. 20 ] - Surgical management of dentigerous cyst and keratocystic odontogenic tumor in children: a conservative approach and 7-year follow-up
- Por que utilizar a associação 30mg do fosfato de codeína e 500mg de paracetamol após exodontias de terceiros molares inferiores impactados?
- Antissepsia de alvéolos pós-exodontia empregando irrigações trans-operatórias de solução de ozônio diluído em água
- Ocorrência de acidentes pérfuro-cortantes em um Curso de Odontologia
- Glycemic control in Type 2 diabetics after minor oral surgery
- Coronectomia de terceiro molar inferior
- Influência do laser terapêutico de baixa potência no tempo de ação da anestesia local após cirurgias odontológicas
- Bisphosphonate-related osteonecrosis of the jaw
- Ozonized water irrigations on dermal wound healing in rats
- Efeito do ozônio diluído em água na morfologia do reparo de feridas dérmicas em ratos induzidos ao Diabetes
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
