Endophytic colonization of potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, pseudomonas putida Strain P9, and its effect on Associated Bacterial Communities (2009)
- Authors:
- USP affiliated authors: AZEVEDO, JOÃO LUCIO DE - ESALQ ; ANDREOTE, FERNANDO DINI - ESALQ
- Unidade: ESALQ
- DOI: 10.1128/AEM.00491-09
- Subjects: BATATA; BACTERIOLOGIA; GENÉTICA VEGETAL
- Language: Inglês
- Abstract: Pseudomonas putida strain P9 is a novel competent endophyte from potato. P9 causes cultivar-dependent suppression of Phytophthora infestans. Colonization of the rhizoplane and endosphere of potato plants by P9 and its rifampin-resistant derivative P9R was studied. The purposes of this work were to follow the fate of P9 inside growing potato plants and to establish its effect on associated microbial communities. The effects of P9 and P9R inoculation were studied in two separate experiments. The roots of transplants of three different cultivars of potato were dipped in suspensions of P9 or P9R cells, and the plants were planted in soil. The fate of both strains was followed by examining colony growth and by performing PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Colonies of both strains were recovered from rhizoplane and endosphere samples of all three cultivars at two growth stages. A conspicuous band, representing P9 and P9R, was found in all Pseudomonas PCR-DGGE fingerprints for treated plantsThe numbers of P9R CFU and the P9R-specific band intensities for the different replicate samples were positively correlated, as determined by linear regression analysis. The effects of plant growth stage, genotype, and the presence of P9R on associated microbial communities were examined by multivariate and unweighted-pair group method with arithmetic mean cluster analyses of PCR-DGGE fingerprints. The presence of strain P9R had an effect on bacterial groupsidentified as Pseudomonas azotoformans, Pseudomonas veronii, and Pseudomonas syringae. In conclusion, strain P9 is an avid colonizer of potato plants, competing with microbial populations indigenous to the potato phytosphere. Bacterization with a biocontrol agent has an important and previously unexplored effect on plant-associated communities
- Imprenta:
- Source:
- Título do periódico: Applied and Environmental Microbiology
- Volume/Número/Paginação/Ano: v. 75, n. 11, p. 3396-3406, june 2009
- Este periódico é de assinatura
- Este artigo é de acesso aberto
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- Cor do Acesso Aberto: green
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ABNT
ANDREOTE, Fernando Dini et al. Endophytic colonization of potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, pseudomonas putida Strain P9, and its effect on Associated Bacterial Communities. Applied and Environmental Microbiology, v. 75, n. 11, p. 3396-3406, 2009Tradução . . Disponível em: https://doi.org/10.1128/AEM.00491-09. Acesso em: 24 abr. 2024. -
APA
Andreote, F. D., Araújo, W. L. de, Azevedo, J. L. de, Elsas, J. D. van, Rocha, U. N. da, & Overbeek, L. S. van. (2009). Endophytic colonization of potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, pseudomonas putida Strain P9, and its effect on Associated Bacterial Communities. Applied and Environmental Microbiology, 75( 11), 3396-3406. doi:10.1128/AEM.00491-09 -
NLM
Andreote FD, Araújo WL de, Azevedo JL de, Elsas JD van, Rocha UN da, Overbeek LS van. Endophytic colonization of potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, pseudomonas putida Strain P9, and its effect on Associated Bacterial Communities [Internet]. Applied and Environmental Microbiology. 2009 ; 75( 11): 3396-3406.[citado 2024 abr. 24 ] Available from: https://doi.org/10.1128/AEM.00491-09 -
Vancouver
Andreote FD, Araújo WL de, Azevedo JL de, Elsas JD van, Rocha UN da, Overbeek LS van. Endophytic colonization of potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, pseudomonas putida Strain P9, and its effect on Associated Bacterial Communities [Internet]. Applied and Environmental Microbiology. 2009 ; 75( 11): 3396-3406.[citado 2024 abr. 24 ] Available from: https://doi.org/10.1128/AEM.00491-09 - Abundance and genetic diversity of nifH gene sequences in anthropogenically affected Brazilian mangrove sediments
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Informações sobre o DOI: 10.1128/AEM.00491-09 (Fonte: oaDOI API)
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