Characterization of nod-like genes in Leishmania (2006)
- Autores:
- Autor USP: ULIANA, SILVIA RENI BORTOLIN - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Idioma: Inglês
- Resumo: Introduction and Objectives: Several proteins of the NOD (nucleotide-binding oligomerization domain) family have been implicated in the intracellular recognition of pathogens and regulation of immune responses. Members of the NOD family in mammals have been associated with the regulation of NF-kB activity and activation of caspases (Annu. Rev. Biochem. 74:355-83, 2005). In the process of studying a genomic region harboring a virulence-related L. amazonensis gene (FEMS Microbiol Lett, 238(1):213-9, 2004), we identified an open reading frame (La17.N3) that presented similarity with the human NOD3 protein. A sequence search in the L. major Friedlin genome allowed the identification of 5 further possible NOD-related genes. One of these genes (LmjF 11.0890) encodes a protein with putative nucleotide-binding domains. The objective of this work is the characterization of 2 of these putative Leishmania NOD-like genes: La17.N3 and LmjF11.0890. Methods and Results: Northern blots indicated that La17.N3 expression is upregulated in L. amazonensis amastigotes, the life cycle stage found in mammalian cells, while LmjF11.0980 seems to be expressed constitutively.The complete nucleotide sequence of La17.N3 was determined and the translated amino acid sequences of La17.N3 and LmjF11.0980 were used to identify orthologous sequences in other Leishmania species. The alignment of these sequences revealed high degrees of similarity. A policlonalantiserum raised against the La17.N3 recombinant protein recognized a 73 kDa protein in total extracts of L. amazonensis and L. major. Constructs for the knock-out and overexpression of the L. amazonensis La17.N3 gene are being concluded. A fragment of 1.2 kb encoding a truncated NOD-like protein LmjF 11.0890 was cloned into a histidine tagged expression vector. The 43-kDa recombinant protein was purified and used to raise specific antibodies. The characterization of this gene and of the encoded protein is under way. Conclusions: The characterization of NOD-like proteins in Leishmania may produce the necessary tools to investigate the participation of these proteins in the macrophage-parasite interaction.
- Imprenta:
- Fonte:
- Título do periódico: Abstracts
- Nome do evento: Meeting of the Brazilian Society for Immunology
-
ABNT
FRANCO, F A L e CRISPIM, A F e ULIANA, Silvia Reni Bortolin. Characterization of nod-like genes in Leishmania. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 19 set. 2024. -
APA
Franco, F. A. L., Crispim, A. F., & Uliana, S. R. B. (2006). Characterization of nod-like genes in Leishmania. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Franco FAL, Crispim AF, Uliana SRB. Characterization of nod-like genes in Leishmania. Abstracts. 2006 ;[citado 2024 set. 19 ] -
Vancouver
Franco FAL, Crispim AF, Uliana SRB. Characterization of nod-like genes in Leishmania. Abstracts. 2006 ;[citado 2024 set. 19 ] - Characterization of Tbmeta1 and 2, T. brucei homologues of the Leishmania infective-stage specific meta proteins.
- Mapping and characterization of the Leishmania (L.) amazonensis genomic region containing the meta 1 gene:: comparative study with the L. (L.) major genome.
- The meta 2 gene of Leishmania: structural characterisation and expression
- Identification of sequences related to the meta 1 gene in trypanosomatids
- Structure and function of a stage-regulated gene in Leishmania
- Caracterização molecular de uma proteína rica em sequencias repetidas de leucina em Leishmania braziliensis
- In Vitro and In Vivo miltefosine susceptibility of a Leishmania amazonensis isolate from a patient with diffuse cutaneous leishmaniasis
- Ros3 (Lem3p/CDC50) gene dosage is implicated in miltefosine susceptibility in Leishmania (Viannia) braziliensis clinical isolates and in Leishmania (Leishmania) major
- Route map for the discovery and pre-clinical development of new drugs and treatments for cutaneous leishmaniasis
- Utilização do gene meta-1 de "Leishmania amazonensis" em ensaios de imunização com DNA
Como citar
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas