Degree of modification of low density lipoprotein (LDL) displays different properties in size and eletric charge and induction of CD11B and CD36 in THP-1 cells (2006)
- Authors:
- USP affiliated authors: NEGRO, SONIA JANCAR - ICB ; GIDLUND, MAGNUS AKE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction: The modifications of lipid and apolipoprotein B components of LDL drive the atherosclerosis process. These modified components can interact and activate macrophages. These properties depend on modifications. These can range from minimal to extensive. The extension/degree of modification and its subsequent Immunobiological properties remains hither to not completely understood. Objectives: In this study we compared the minimally oxidative modification (mmLDL) and highly oxidated LDL (HoxLDL) induced by iron or copper respectively, with the objective to detect differences with respect to biochemistry and immunobiological properties. Methods: LDL was prepared by ultracentrifugation. The oxLDL was prepared by addition of CuSO4 (20 µM 18h, 37 ºC) or FeSO4 (1 µM for 48 h RT). One part was labeled by FITC. To analyze the biochemical properties (size, charge) we used size exclusion (Superdex 200R) and anions exchange chromatography (Resource-Q, NaCl gradient 0- 500 mM) respectively using a FPLC system. Then we compared the uptake by THP-1 (using the FITC labeled). The change of expression of CD36, CD11b, CD32 (FcãRII) and CD128 (CXCR2) was analyze by FACS. Results: Table: Differences between mmLDL and oxLDL.....
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
RIOS, F J O et al. Degree of modification of low density lipoprotein (LDL) displays different properties in size and eletric charge and induction of CD11B and CD36 in THP-1 cells. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 28 mar. 2024. -
APA
Rios, F. J. O., Jancar, S., Melo, I. B., Pereira, F. Z., & Gidlund, M. A. (2006). Degree of modification of low density lipoprotein (LDL) displays different properties in size and eletric charge and induction of CD11B and CD36 in THP-1 cells. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Rios FJO, Jancar S, Melo IB, Pereira FZ, Gidlund MA. Degree of modification of low density lipoprotein (LDL) displays different properties in size and eletric charge and induction of CD11B and CD36 in THP-1 cells. Abstracts. 2006 ;[citado 2024 mar. 28 ] -
Vancouver
Rios FJO, Jancar S, Melo IB, Pereira FZ, Gidlund MA. Degree of modification of low density lipoprotein (LDL) displays different properties in size and eletric charge and induction of CD11B and CD36 in THP-1 cells. Abstracts. 2006 ;[citado 2024 mar. 28 ] - Oxidized low density lipoprotein inhibits LPS/IFN-induced nitric oxide production in RAW264.7 cells - effects of a PAF-antagonist
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- Vascular permeability is increased by oxidized low density lopoprotein (oxLDL) and selected apolipoprotein B derived synthetic polypeptides
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- Increased microvascular permeability in the hamster cheek pouch induced by oxidized low density lipoprotein (oxLDL) and some fragmented apolipoprotein B proteins
- Role of PPAR-gamma in the modulation of CD36 and FcgammaRII induced by LDL with low and high degrees of oxidation during the differentiation of the monocytic THP-1 cell line
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