Cytotoxic effects of cinnamic acid on melanoma cells in culture (2004)
- Authors:
- Autor USP: SANTELLI, GLAUCIA MARIA MACHADO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: Cinnamic acid (CA) is a naturally occurring aromatic fatty acid that has a long history of human exposure as a component of plant-derived scents and flavoring. Its effects include antimicrobial and anti-inflammatory activity. The present work propose to evaluate the CA cytotoxical potential on human melanoma cell line ,HT-144. Cell viability was determined by using MTT essay after 48h cell treatment with several drug concentrations. The IC50 was established as 2,40mM. Further, studies on the probable CA mechanisms of action were performed with two concentrations considered as mild and drastic, 2.20 and 3.24mM respectively. Alterations in the pattern of organization of microtubules were analyzed in cytological preparation stained by imunofluorescence with anti-? tubulin + anti-? tubulin monoclonal antibodies. Tubulin bundles seem to dissociate after 48h treatment with 3.24mM of CA. Microfilaments were also desorganized with both concentrations of the drug. This effect was visualized by imunofluorescence with phalloidin. The effects seem to increase the frequency of binucleated cells in HT-144 culture. The number of binucleated cells increased from 1.07% to 1.60% in cells treated with 0.20mM of CA and to 3% with 3.24mM. DNA quantification by image analysis did not show alteration in DNA indexes after CA treatment. Nevertheless, the M30 staining showed that the treatment caused an increase in the apoptotic cells frequency
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
NIERO, Evandro Luís de Oliveira e MACHADO-SANTELLI, Gláucia Maria. Cytotoxic effects of cinnamic acid on melanoma cells in culture. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 19 abr. 2024. -
APA
Niero, E. L. de O., & Machado-Santelli, G. M. (2004). Cytotoxic effects of cinnamic acid on melanoma cells in culture. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Niero EL de O, Machado-Santelli GM. Cytotoxic effects of cinnamic acid on melanoma cells in culture. Abstracts. 2004 ;[citado 2024 abr. 19 ] -
Vancouver
Niero EL de O, Machado-Santelli GM. Cytotoxic effects of cinnamic acid on melanoma cells in culture. Abstracts. 2004 ;[citado 2024 abr. 19 ] - Cytoskeleton and nuclear DNA content in pleomorphic cell cultures
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