Labeling pattern of M30 antibody during apoptotic process induced by UV radiation (2004)
- Autores:
- Autor USP: SANTELLI, GLAUCIA MARIA MACHADO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Idioma: Inglês
- Resumo: The present study focused the dynamic of apoptosis process in monolayer cell culture. The monoclonal antibody M30, specific for the neo-epitope in cytokeratin 18 that becomes available at an early caspase cleavage event during apoptosis, can be utilized specifically to recognize apoptotic epithelial cells. Indeed, cytoskeleton proteolytic cleavage occurs before DNA strand breaking and plasma membrane blebbing. The ultraviolet (UV) radiation is an environmental agent that leads to apoptosis in epidermal cells, probably as a process to rid potentially cancerous cells originated by mutagenic UV irradiation. The BBnt (normal epithelial bovine cell bladder) cells showed a high level of apoptosis induction (77,6% of cells) 24 hours after UV radiation for 1 minute. Typical nuclear apoptotic morphology was observed in those cells when they were stained with propidium iodide or DAPI. Immunofluorescence using M30 antibody was performed to detect early apoptosis phases in the monolayer cells compared with those in suspension. M30 labeling pattern analyzed by confocal microscopy was associated with the kinetics of apoptosis induction
- Imprenta:
- Fonte:
- Título do periódico: Abstracts
- Nome do evento: Congress of the Brazilian Society for Cell Biology
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ABNT
NAKAGAWA, Elly Kayoko et al. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 28 mar. 2024. -
APA
Nakagawa, E. K., Urabayashi, M. S., Cortez, B. de A., & Machado-Santelli, G. M. (2004). Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Nakagawa EK, Urabayashi MS, Cortez B de A, Machado-Santelli GM. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. Abstracts. 2004 ;[citado 2024 mar. 28 ] -
Vancouver
Nakagawa EK, Urabayashi MS, Cortez B de A, Machado-Santelli GM. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. Abstracts. 2004 ;[citado 2024 mar. 28 ] - Cytoskeleton and nuclear DNA content in pleomorphic cell cultures
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