Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines (2003)
- Authors:
- Autor USP: FERREIRA, LUIS CARLOS DE SOUZA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Expression of heterologous proteins by recombinant Bacilllus subtilis strains represents an alternative method for the development of safe mucosal vaccines against enteric and systemic pathogens. In this work we report the construction of recombinant B. subtilis strains able to express two proteins derived from enterotoxigenic Escherichia coli strains, one of the most important ethiological agent of infectious diarrhoea either for children or travellers in developing countries. Genes encoding the b-subunit of the heat-labile toxin (LTB) and the structural subunit of the colonization factor antigen (CFAB) were amplified by specific primers and cloned in the integration vetor pX, able to control expression of heterologous genes following addition of different concentrations of xylose in the growth medium. The recombinant plasmids were constructed in E. coli strains and transferred by transformation to naturally competent B. subtilis cells. Recombinant clones were selected for the integration of the expression cassettes at the chromosomal amyE locus, previously interrupted by a neomycin resistance-encoding gene. Allelic exchanges generated by two crossing over events were selected by the loss of the neomycin resistance marker and acquisition of chloramphenicol resistance encoded by the recombinant gene cassette. B. subtilis able to express either CFAB or LTB were in vitro analyzed with Western blot following incubation of the recombinant strains in growth mediumadded with 0.8% xylose for different periods. The recombinant B. subtilis strains were shown to express stably the ETEC antigens without the need of any selective pressure. Furthermore, the immunogenicity of the ETEC-B. subtilis bivalent strains were evaluated following oral or parenteral delivery of live bacterial cells to BALB/c mice
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference titles: Congresso Brasileiro de Microbiologia
-
ABNT
PACCEZ, Juliano Domiraci et al. Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines. 2003, Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. . Acesso em: 20 abr. 2024. -
APA
Paccez, J. D., Luiz, W. B., Schumann, W., & Ferreira, L. C. de S. (2003). Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines. In . Sociedade Brasileira de Microbiologia. -
NLM
Paccez JD, Luiz WB, Schumann W, Ferreira LC de S. Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines. 2003 ;[citado 2024 abr. 20 ] -
Vancouver
Paccez JD, Luiz WB, Schumann W, Ferreira LC de S. Expression of enterotoxigenic Escherichia coli proteins by genetically modified Bacillus subtilis strains as a new approach to develop mucosal vaccines. 2003 ;[citado 2024 abr. 20 ] - Avaliação das propriedades imunológicas e papel vacinal da flagelina fliCi de Salmonella enterica pv Typhimurium
- Immunogenicity of flagelins FliCi e FliCd following oral immunization of mice with attenuated salmonella strains
- Evaluation of the genetic diversity among enterotoxigenic Escherichia coli (ETEC) strains isolated from humans in Brazil and Argentina
- Construção de uma vacina de DNA bivalente contra os vírus do papiloma e herpes simplex humano
- A DNA vaccine encoding the enterohemorragic Escherichia coli shiga-like toxin 2 'A ind.2' and B subunits confers protective immunity to shiga toxin challenge in the murine model
- Produção e purificação da flagelina FliCi de S. Typhimurium
- Surface expression of adhesins enhances the vaccine potential of orally delivered Bacillus subtilis strains
- Immune responses and therapeutic antitumor effects of an experimental DNA vaccine encoding human Papillomavirus type 16 oncoproteins genetically fused to Herpesvirus glycoprotein D
- Characterization of a DNA vaccine encoding the HPV-16 E7 oncoprotein genetically fused to the N-terminal domain of Salmonella enterica SV Müenchen FLICd flagellin
- Biotecnologia aplicada ao desenvolvimento de vacinas
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