Identification and cloning of the oligopeptide binding protein (OPPA) Xanthomonas axonopodis pv citri (2003)
- Autores:
- Autores USP: FERREIRA, RITA DE CASSIA CAFE - ICB ; FERREIRA, LUIS CARLOS DE SOUZA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Idioma: Português
- Resumo: Uptake of oligopeptides with more than three amino acids is mediated by the oligopeptide uptake system, an ABC-type transport system composed of four membrane-bound docking elements (OppB, OppC, OppD, OppF) and one binding periplasmic component, the oligopeptide binding protein (OppA), which confer substrate affinity and specificity to the transport system. In this work the oppA orthologue of the plant pathogen Xanthomonas axonopodis pv. citri were cloned and expressed in Escherichia coli K12 BL21 cells using the pET28 and pET29 expression vectors. Recombinant proteins fused to poly-histidine tag sequences at both N- and C-terminal regions of the OppA protein were expressed at high levels by E. coli K12 strains transformed with the engineered plasmid vectors.All proteins were expressed as inclusion bodies in the cytoplasm of the recombinant proteins, which were purified either by affinity chromatography with Ni-containing resin or ion-exchange chromatography following solubilization of the insoluble proteins. Specific anti-OppA antibodies were raised in mice immunized with the purified recombinant OppA proteins and used to monitor expression of the OppA in periplasm-enriched fractions of X. auxonopodis pv citri during in vitro growth. Further analysis of the Xanthomonas OppA may help to define substrate affinity and specificity of this protein
- Imprenta:
- Editora: Comissão de Pesquisa do ICB/USP
- Local: São Paulo
- Data de publicação: 2003
- Fonte:
- Título do periódico: Resumos
- Nome do evento: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
PACCEZ, Juliano Domiraci et al. Identification and cloning of the oligopeptide binding protein (OPPA) Xanthomonas axonopodis pv citri. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 25 abr. 2024. -
APA
Paccez, J. D., Ferreira, R. de C. C., Ferreira, L. C. de S., Suzuki, C. F., & Balan, A. (2003). Identification and cloning of the oligopeptide binding protein (OPPA) Xanthomonas axonopodis pv citri. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Paccez JD, Ferreira R de CC, Ferreira LC de S, Suzuki CF, Balan A. Identification and cloning of the oligopeptide binding protein (OPPA) Xanthomonas axonopodis pv citri. Resumos. 2003 ;[citado 2024 abr. 25 ] -
Vancouver
Paccez JD, Ferreira R de CC, Ferreira LC de S, Suzuki CF, Balan A. Identification and cloning of the oligopeptide binding protein (OPPA) Xanthomonas axonopodis pv citri. Resumos. 2003 ;[citado 2024 abr. 25 ] - Bacillus subtilis endospores at high purity and recovery yields: optimization of growth conditions and purification method
- Purification and in vitro characterization of the maltose-binding protein of the plant pathogen Xanthomonas citri
- Bacillus subtilis expression vectors capable of expressing heterologous proteins at different cellular compartments
- Construção de um novo sistema de expressão e purificação de proteínas recombinantes baseada em esporos de Bacillus subtilis
- Boosting systemic and secreted antibody responses in mice orally immunized with recombinant Bacillus subtilis strains following parenteral priming with a DNA vaccine encoding the enterotoxigenic Escherichia coli (ETEC) CFA/I fimbriae B subunit
- Generation of specific polyamine uptake knockout mutants of Streptococcus mutans UA159 strain
- Streptococcus mutans glutamate binding protein (GlnH) as antigen target for a mucosal anti-caries vaccine
- Antibody Responses Elicited In Mice Immunized With Bacillus Subtilis Vaccine Strains Expressing STX2B Subunit of Enterohaemorragic Escherichia Coli O157:H7
- Molybdate uptake system of Xanthomonas axonopodis pv. Citri contributes to virulence symptoms in grapefruit
- Immune modulatory effects of the Mycobacterium bovis GroEL2 expressed by genetically modified Baccillus subtilis strains employed as delivery vectors of vaccine antigens
Como citar
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
