Molecular identification of G[9]P[8] genotypes of human rotavirus in São Paulo, Brazil (2001)
- Autores:
- Autor USP: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB
- Unidade: ICB
- Assuntos: CIÊNCIAS (CONGRESSOS;CONFERÊNCIAS); GENÓTIPOS (IDENTIFICAÇÃO); ROTAVIRUS; GASTROENTEROPATIAS
- Idioma: Inglês
- Resumo: Rotaviruses have been considered the most important cause of gastroenteritis in young children around the world. The two capsid proteins, VP7 and VP4 are used for molecular characterization of genotypes G and P, respectively. Fecal samples collected in São Paulo, Brazil from December 1995 to November 1999, were screened for rotavirus with EIARA Fiocruz Kit. From a total of 236 samples, 44(18,6%) were positive for rotavirus in EIARA.Objectives: The bjective of this work was to chacterization this samples by RT-PCR. Methods and Results: Preliminary results of genotypes G characterization by RT-PCR using a pool of primers for G1, G2, G3, G4 and G9 in 11 positive samples resulted in 3 samples with G[9] specificity. These results were confirmed by using the G[9] specific primer 9T-9B alone. These 11 samples were also tested by RT-PCR for genotype P characterization and the same 3 G[9] samples were characterized as P[8]. Conclusions: The unusual G[9] genotype detected for the first time in São Paulo, Brazil suggests, as many others studies worldwide, that genotype G[9] viruses may be widely distributed and more important than previously recognized
- Imprenta:
- Editora: Comissão de Cultura e Extensão Universitária do ICB/USP
- Local: São Paulo
- Data de publicação: 2001
- Fonte:
- Título do periódico: Resumos
- Nome do evento: Congresso do Instituto de Ciências Biomédicas
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ABNT
SCHMICH, H. et al. Molecular identification of G[9]P[8] genotypes of human rotavirus in São Paulo, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 23 abr. 2024. -
APA
Schmich, H., Rácz, M. L. B. de O., Fagundes Neto, U., & Munford, V. (2001). Molecular identification of G[9]P[8] genotypes of human rotavirus in São Paulo, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Schmich H, Rácz MLB de O, Fagundes Neto U, Munford V. Molecular identification of G[9]P[8] genotypes of human rotavirus in São Paulo, Brazil. Resumos. 2001 ;[citado 2024 abr. 23 ] -
Vancouver
Schmich H, Rácz MLB de O, Fagundes Neto U, Munford V. Molecular identification of G[9]P[8] genotypes of human rotavirus in São Paulo, Brazil. Resumos. 2001 ;[citado 2024 abr. 23 ] - Sequence analysis of VP4 gene of porcine rotavirus strains displaying human P[6] genotype specificity
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