Occurrence of adenoviruses in waste water samples in São Paulo city. Brazil (2001)
- Autores:
- Autores USP: MEHNERT, DOLORES URSULA - ICB ; HARSI, CHARLOTTE MARIANNA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Idioma: Inglês
- Resumo: In Brazil, sewage is commonly discharged without treatment into creeks and rivers, especially in the suburban areas. About 30 millions of people have no access to treated drinking water and only a limited segment of the population in the large cities is served by an urban sewage system. Adenoviruses are the second cause of viral gastroenteritis in children in Brazil and no data are available about the circulation of such viruses in the environment. A total of 43 samples of raw sewage and sewage-polluted creek water were collected at two different sites in city of São Paulo through July 1998 to June 1999: Sewage Pumping Station Edu Chaves and Creek Pirajussara, located on north and south districts of the city, respectively. Four-liter samples were submitted to a two-step concentration procedure based on filtration through a microporous electropositive charged filter membrane (Zeta Plus 60S) and ultracentrifugation. Adenoviruses were detected by PCR using the primers, described previously by Allard et al. (1990), for the amplification of fragments of 300 bp and 143 bp of the hexon gene. Viruses were also passaged, no more than three times, in Hep-2 cells and then submitted to PCR. Adenoviruses were detected in 25 samples (58.1%) out of 43 examined, most of them only after a reamplification reaction, suggesting that these viruses are present in sewage and creek water through out the year at low concentration
- Imprenta:
- Editora: Comissão de Cultura e Extensão Universitária do ICB/USP
- Local: São Paulo
- Data de publicação: 2001
- Fonte:
- Título do periódico: Resumos
- Nome do evento: Congresso do Instituto de Ciências Biomédicas
-
ABNT
SANTOS, M F et al. Occurrence of adenoviruses in waste water samples in São Paulo city. Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 19 abr. 2024. -
APA
Santos, M. F., Pauli, V., Sassaroli, A., Garrafa, P., Mosca, X., Mehnert, D. U., et al. (2001). Occurrence of adenoviruses in waste water samples in São Paulo city. Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Santos MF, Pauli V, Sassaroli A, Garrafa P, Mosca X, Mehnert DU, Vieira MJ, Hársi CM. Occurrence of adenoviruses in waste water samples in São Paulo city. Brazil. Resumos. 2001 ;[citado 2024 abr. 19 ] -
Vancouver
Santos MF, Pauli V, Sassaroli A, Garrafa P, Mosca X, Mehnert DU, Vieira MJ, Hársi CM. Occurrence of adenoviruses in waste water samples in São Paulo city. Brazil. Resumos. 2001 ;[citado 2024 abr. 19 ] - Distribution of rotavirus G1-G5 genotypes in domestic effluent and creek in São Paulo city from 2000 to 2001
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