Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes (2001)
- Autores:
- Autores USP: MEHNERT, DOLORES URSULA - ICB ; HARSI, CHARLOTTE MARIANNA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Idioma: Inglês
- Resumo: A two-step virus concentration method has been routinely used in our lab for rotavirus detection in raw sewage and surface water samples. The application of such method for monitoring viruses in environmental samples is relatively simply. The first concentration step, based on filtration through electropositive filter membrane, can be done in loco, but the reconcentration one is restricted to a few laboratories due to the use of a ultracentrifuge. To enabling the use of this method in most of the brazilian counties, the filter membrane should be mailed by post to the nearest reference laboratory for elution and reconcentration of viruses. In order to evaluate for how long the rotaviruses resist adsorbed to a microporous filter membrane without significant loses of their infectivity, simian rotaviruses (104 FFU/50ml) were experimentaly seeded in one-liter of creek water and recovered by the filtration through Zeta Plus 60S filter membrane. The membranes were wrapped on plastic and stored at 4oC. The adsorbed viruses were eluted with beef-extract 3%-glicyne 0.05 M pH 9.0 after 6, 18, 24 and 48 h. Rotaviruses eluted immediately after filtration (t0) were considered as positive controls. Results show that rotaviruses can be recovered from positively charged membranes after 48h without significant loses of infectivity so that the membranes used for virus concentration can be mailed by post within this period
- Imprenta:
- Editora: Comissão de Cultura e Extensão Universitária do ICB/USP
- Local: São Paulo
- Data de publicação: 2001
- Fonte:
- Título do periódico: Resumos
- Nome do evento: Congresso do Instituto de Ciências Biomédicas
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ABNT
GARRAFA, P. et al. Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 23 abr. 2024. -
APA
Garrafa, P., Pauli, V., Mehnert, D. U., Vieira, M. J., & Hársi, C. M. (2001). Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Garrafa P, Pauli V, Mehnert DU, Vieira MJ, Hársi CM. Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes. Resumos. 2001 ;[citado 2024 abr. 23 ] -
Vancouver
Garrafa P, Pauli V, Mehnert DU, Vieira MJ, Hársi CM. Resistence of rotaviruses adsorbed to zeta plus 60s filter membranes. Resumos. 2001 ;[citado 2024 abr. 23 ] - Distribution of rotavirus G1-G5 genotypes in domestic effluent and creek in São Paulo city from 2000 to 2001
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