Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane (2000)
- Autores:
- Autor USP: TULMANN NETO, AUGUSTO - CENA
- Unidade: CENA
- DOI: 10.1007/s002990000253
- Assuntos: CANA-DE-AÇÚCAR; HERBICIDAS; PLANTAS CULTIVADAS; PLANTAS PRODUTORAS DE AÇÚCAR
- Idioma: Inglês
- Resumo: Embryogenic calli of the Brazilian sugarcane (Saccharum officinarum L.) genotype SP80-180 were transformed with two plasmids containing genes coding for neomycin phosphotransferase (neo) and phosphinotrycin acetyltransferase (bar), by particlebombardment using an apparatus developed at Copersucar Technology Center. Transformed plants were initially selected on culture medium containing Geneticin, and resistance was confirmed by localized application of a kanamycin solution to leavesof hardened plants at the nursery. A commercial formulation of ammonium gluphosinate was sprayed twice on these antibiotic-resistance plants. The resistance plants were considered co-transformed, and Southern analysis confirmed stableintegration of both bar and neo genes. In addition, phosphinotrycin acetyltransferase expression was supported by RT-PCR analysis and neomycin phospho-transferase presence was demonstrated by western blotting. Simillar analyses were alsoperformed with micropropagated transformants after three cycles of subculture
- Imprenta:
- Fonte:
- Título do periódico: Plant Cell Reports
- Volume/Número/Paginação/Ano: v.19, p.1188-1194, 2000
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
FALCO, M C e TULMANN NETO, Augusto e ULIAN, E C. Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane. Plant Cell Reports, v. 19, p. 1188-1194, 2000Tradução . . Disponível em: https://doi.org/10.1007/s002990000253. Acesso em: 17 abr. 2024. -
APA
Falco, M. C., Tulmann Neto, A., & Ulian, E. C. (2000). Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane. Plant Cell Reports, 19, 1188-1194. doi:10.1007/s002990000253 -
NLM
Falco MC, Tulmann Neto A, Ulian EC. Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane [Internet]. Plant Cell Reports. 2000 ;19 1188-1194.[citado 2024 abr. 17 ] Available from: https://doi.org/10.1007/s002990000253 -
Vancouver
Falco MC, Tulmann Neto A, Ulian EC. Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane [Internet]. Plant Cell Reports. 2000 ;19 1188-1194.[citado 2024 abr. 17 ] Available from: https://doi.org/10.1007/s002990000253 - Indução de mutação e seleção in vitro de mutantes tolerantes a salinidade utilizando Nicotiana tabacum como planta modelo
- Brasil: fitomejoramiento del arroz (Oriza sativa L.)
- Confirmada tolerancia ao virus do mosaico do mamoeiro em progenies de IAC-98
- Uso de cultura de tecidos em bananeira maca visando inducao de mutações através de raios gama
- Genetic improvement of beans (Phaseolus vulgaris L.) through mutations induction
- Variacao somacional em bananeira: um estudo de caso
- Induction of shrubby type mutant in Stylosanthes guyanensis
- Study of mating system of sweet orange (citrus sinensis l. Osbeck)
- Mutation breeding in Stromantha sanguinea cv sanguinea by gamma radiation
- Use of gamma-rays in mutation breeding of calathea louisae (cv. Albertii)
Informações sobre o DOI: 10.1007/s002990000253 (Fonte: oaDOI API)
Como citar
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
